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Cell concentrations were measured using a glass hemocytometer and coverslip daily. 2.4. The Establishment of NASH Rat Model and Animal Groups Eight Sprague-Dawley rats were fed with a normal diet as a control group (Table 1). Forty-eight Sprague-Dawley rats were fed with high-fat diet (...
The temperature was maintained constant by circulating refrigerated or heated water through the jacket of the glass jacketed beaker. The circulating water was cooled/heated using a thermostatized bath (Tecnal Model TE-184, Tecnal, Piracicaba, Brazil). All experiments were carried out in duplicate....