目的:检测中空纤维丝、热可逆凝胶和低吸附培养板3种三维培养体系中丙型肝炎病毒(HCV)增殖水平,寻找效率较高的HCV体外培养系统。方法:利用中空纤维丝、热可逆凝胶及低吸附培养板培养永生化人肝细胞HuS-E/2,通过XTT法检测细胞增殖情况。以基因型为2a型的HCV病毒株JFH1进
JFH-1 BASED HCV CELL CULTURE SYSTEMS FOR NS5A OF GENOTYPES 1-7The present inventors developed hepatitis C virus recombinants expressing NS5A from genotype 1a, 1b, 2a, 3a, 4a, 5a, 6a or 7a in the context of a genotype 2a backbone. Additional recombinants express NS5A and the structural ...
发现目前在实验室里广泛使用的毒株JFH1的Takaji Wakita,建立HCV复制子模型从而用于筛选药物的Ralf Bartenschlager,建立HCV细胞感染模型从而可以研究整个病毒生命周期的Prof. Zhong (Francis Chisari实验室),和研发出Sofosbuvir的Michael Sofia。每个人的工作都值得铭记。
Cyclosporine A (CsA) is a well-characterized anti-HCV reagent. Recently it was reported that the genotype 2a JFH-1 strain was more resistant than genotype 1 HCV strains to CsA in a cell culture system. However, the JFH-1 responsible region for the resistance to CsA remains unclear. It was...
研究热和紫外线照射对HCV的灭活效果.方法 感染性滴度为2.5×104 Ffu/ml的丙型肝炎病毒JFH-1株(HCV JFH-1 strain)原液,经56℃水浴或紫外线照射不同时间后,感染Huh7-25-CD81细胞系,应用间接免疫荧光法测定病毒感染性滴度的动态变化.若被感染的细胞经旨传3代后,用问接免疫荧光法检测为阴性,则...
Exosomes from HCV J6/JFH-1 infected Huh7.5 cells and HCV patients mediate effective HCV transmission in the presence of anti-CD81, anti-SB-RI or anti-APOE antibody.Terence N. BukongFatemeh MomenHeraviKaren KodysShashi ...
To facilitate studies of hepatitis C virus (HCV) NS4A, we aimed at developing J6/JFH1-based recombinants with genotype 1- to 7-specific NS4A proteins. We developed efficient culture systems expressing NS4A proteins of genotypes (isolates) 1a (H77 and TN), 1b (J4), 2a (J6), 4a (ED...
A number of studies have suggested that the outcome of HCV infection as well as the response to interferon treatment depends on the genotype or quasispecies with which the patient is infected. However, it is not clear how subtle differences in the HCV genome affect viral replication, infectivity...
Background & Aims: Recently, full viral life cycle hepatitis C virus (HCV) cell culture systems were developed for strain JFH1 (genotype 2a) and an intragenotypic 2a/2a genome (J6/JFH). We aimed at exploiting the unique JFH1 replication characteristics to develop culture systems for genotype...
More than 70 million people are estimated to be chronically infected with HCV, however, the infection status has been established in < 20% of these individuals. Annually, over 2 million new acute infections occur, with a chronicity rate of > 70%. This poses significant scientific ...