(a) Endonuclease activities of purified recombinant CRT1, its truncated mutant CRT1-CC and Nb.BsrDI, a positive control, were determined in the presence of 10 mM MnCl2, MgCl2 or ZnCl2. Following incubation with plasmid DNA, supercoiled (SC) and relaxed (RL) DNA was resolved on a 1.2% ...
personal motions & expressions-under diverse conditions, including continuous aqueous exposures, physical abrasions etc.We show a disposable aqueous major battery pack biochemistry that comprises environmentally harmless materials regarding the sulfur cathode and Zn anode in a 1 M ZnCl2 aqueous electrolyte....
a Friedrichcondenser, and a nitrogen-inlet tube is flushed with nitrogen, and then charged with asuspension of lithium aluminum hydride (47.9 g, 1.26 mol) in 1200 mL of tetrahydrofuran(THF). The mixture is cooled (10°C, ice bath) and L-valine (100 g, 0.85 mol) is added inportions o...
The channels open transiently at first, causing a characteristic noise in membrane potential difference (PD), and after longer exposure remain open with a typical current- voltage (I/V) profile, both abolished by the addition of 1 mM ZnCl2, the main known blocker of animal H+ channels. The...
To capture the ZBTB43 fraction, the fragmented mouse genomic DNA was mixed with the pre-incubated beads in a 200 µl reaction volume of binding buffer (10 mM Tris-HCl pH 7.8, 100 mM NaCl, 10 mM MgCl2, 0.05% NP40, 25 ng/µl BSA, 1 mM DTT, 0.05 mM ZnCl2) ...
What mass of silver chloride can be precipitated from a silver nitrate solution by 200 mL of a solution of 0.50 M CaCl2? Equal volumes of 0.240 M AgNO3 and 0.140 M ZnCl2 solution are mixed. Calculate the equilibrium concentrations of Ag+ and Zn2+. What mass of N...
What is the net ionic equation for the reaction of ZnCl2 with NaOH? Provide a balanced equation for the reaction between NaOH and HCl. a. Write a balanced chemical equation for the reaction of phosphoric acid with sodium hydroxide. b. Calculate the molarity of a phosphoric acid solution i...
以二甲酚橙为指示剂,用0.1024m01/L的EDTA溶液25.00mL滴定至终点,则氯化锌的含量为()M(ZnCl2)=136.29g/mol A. 0.9692 B. 0.969 C. 0.4846 D. 0.485 查看完整题目与答案 保留两位小数约是__,精确到十分位约是__. 查看完整题目与答案 感知觉是婴儿认识世界和自己的基本手 A. 正确 B. 错误 ...
ZnCl2, 5% glycerol) supplemented with 1 mM DTT and 35 mM imidazole. For the final wash step, DTT was omitted from the wash buffer. Protein was eluted with CTCF buffer supplemented with 300 mM imidazole. The eluate was subsequently concentrated approximately twofold using a Sartorius ...
硼氢化钠通常不能用来直接还原羧酸,但是Lewis 酸的存在可大大提高其还原能力,从而用于羧酸的还原。由于LAH 和硼烷的价格相对要贵,因此工业上大量生产时,一般都应用该方法,比较常用的体系为:NaBH4-BF3, NaBH4-ZnCl2. J. Org. Chem., Vol. 63, No. 12, 1998. ...