How does the lac operon work? The lac operon is required for the transport and metabolism of lactose. The lacI gene is constitutively expressed, meaning that it is continuously transcribed by cells whether lactose is present or not. In the absence of lactose, the LacI protein binds to the pr...
Although rhaR is divergently transcribed from the adjacent lraC gene in A. nidulans (and other filamentous fungi; Additional file 1: Figure S1B), rhaR and lraC are not co-regulated: whereas expression of rhaR is constitutive that of lraC is induced when l-rhamnose is present as sole ...
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res., 9 (1981), pp. 6505-6525 Google Scholar 39 TB van Dijk, E Caldenhoven, JA Raaijmakers, et al. Multiple tyrosine residues in the intracellular domain of the common beta subunit...
Another example of a cis gene is the operator gene in lac operon. It when bound by the repressor molecule is unable to initiate transcription. However, when the inducer (lactose) binds to the repressor molecule inactivating it, normal transcription can occur. Share Possibly...
Removal of one half of the operator prevented QacR-mediated repression of the qacA promoter. Purified QacR protein bound specifically to this operator sequence in DNase I-footprinting experiments. Importantly, addition of diverse QacA substrates was shown to induce qacA expression in vivo, as ...
Reviewing Vocab… GENE: a segment of DNA that codes for a particular protein/trait An expressed gene is one that is being transcribed (i.e. it is turned to the “on” position and is being used to make proteins!) A repressed gene is one that is not being transcribed (i.e. it is ...
Although it has become routine to consider DNA in terms of its role as a carrier of genetic information, it is also an important contributor to the control of gene expression. This regulatory principle arises from its structural properties. DNA is maintained in an underwound state in most bacte...
Plasmid pFAT595 harbors the tatD gene under the control of the UV5 promoter/lac operator on plasmid pQE60 (Qiagen) and was constructed as follows. The tatD gene and flanking sequence was amplified by PCR using pFAT65 as template and primers 5′-GCGCGAATTCCGTCAGGGCGGTTGTCATATGGAG-3′ an...
We opted to be part of the case study because WP is uniquely positioned in the market as the largest global OWF developer and operator. We also thought the case to be interesting because DONG Energy itself is a Denmark-based, government-owned utility company going through a major strategic ...
E. coli DH5α ∆lac was transformed with pairs of plasmids: pCM132m/pKGB8 (empty vectors as a control); pCM132m/pKKB2.11 (araBADp-PA3458); pKKB2.31 (PA3458p-lacZ)/ pKKB2.11; pKKB2.31/pKGB8; pKKB2.32 (PA3459p-lacZ)/pKKB2.11; pKKB2.32/pKGB8. The β-galactosidase activity ...