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Isocratic elution was performed with acetonitrile/methanol/H2O (9:7:84, v/v) as mobile phase at a flow rate of 0.3 ml min À 1 and fluorescence was monitored with a fluorescence detector (RF-10A XL, Shimadzu) at 372 nm for excitation and 456 nm for emission. For the assignment of ...
Since Bcl-xl does not interact with parkin in mammalian cells,32 we swapped the full a-helix 2 of Bax containing the BH3 domain with the complete a-helix 2 of the anti-apoptotic Bcl-xl containing its BH3 domain (Bax- BH3sw) in an effort to preserve proper folding (Figure 5a). We...
parent ion mass of DMB-labelled mono-O-acetylated Neu5Ac and a fragmentation pattern characteristic for DMB-Neu5,9Ac2(ref.45;Fig. 3f,g). Together, these data demonstrate that the isolated SGNH-like domain of CASD1 displays SOAT activityin vitroand transfers acetyl groups from acetyl-CoA to...