A reversible reaction is achemical reactionwhere thereactantsformproductsthat, in turn, react together to give the reactants back. Reversible reactions will reach an equilibrium point where the concentrations of the reactants and products will no longer change. A reversible reaction is denoted by ...
a此步反应是可逆的。然后蔗糖磷酸酯酶(SPP) 将蔗糖-6-P迅速降解为蔗糖和磷酸根离子, 而SPS 和SPP 是以复合体的形式存在植物体内, 因此SPS 催化蔗糖生成实际是不可逆的合成过程(Huber and Huber,1996)。 This step response is reversible.Then sucrose phosphatase (SPP) sucrose - 6-P rapid degeneration for...
Treatment of 3,3-diphenyl-1-propanol under cobalt-catalyzed hydroformylation conditions gives the corresponding aldehyde as the principal product but a small quantity of 1,1-diphenylethylene is also formed. Similarly 3-phenyl-1-propanol gives a small quantity of styrene. These arylethylenes are ...
CFSs of tested strains were 100-fold diluted in 0.1 M HCl, sonicated, then diluted 1000 times by deionized water to fit into the calibration curve, and sterile-filtered through a syringe filter (0.22 μm). 5 µl was injected for LC-MS/MS analysis. ...
Genomic DNA was isolated using a cetyltrimethylammonium bromide (CTAB)-based phenol-chloroform extraction as previously described5. Pelleted cells were resuspended in sodium dodecyl sulfate-Edward’s Buffer (SDS-EB; 20 mM Tris/HCl, 10 mM EDTA, 100 mM NaCl, 2% SDS) and incubated with ...
Testes were lysed in Pierce IP lysis buffer (Thermo Scientific, no.87787, 25 mM Tris HCl, pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, and 5% glycerol) containing protease inhibitors (Roche, mini-complete, no EDTA) and homogenized. The lysates were vortexed and spun at 17,000...
16 The reaction used to manufacture ammonia from nitrogen and hydrogen is reversible.An equilibrium can be established between ammonia, nitrogen and hydrogen.Which statement describes the equilibrium? A Both the forward reaction and the backward reaction have the same rate. B The rate of the ...
Then the cells were incubated at 37 °C for 14 h with 100 µL of 0.1 g/ml SDS (H5113, Promega, Madison, WI) in 0.1M HCl to dissolve formazan formed in cells after the reaction with MTT. The absorbance of formazan in each well was measured at 570 nm using Epoch microplate ...
The cell pellet was re-suspended in 20 ml lysis buffer [20 mM Tris-HCl pH 8.0, 500 mM NaCl, 0.1% (v/v) Triton] supplemented with the complete protease-inhibitor mix (Roche) and lysed by ultrasonication (Misonix XL2020; Misonix, USA) for 10 seconds with 15 seconds pulse ...
for 18 h. Cells were pelleted at 2,415g and 4 °C, and were subsequently re-suspended in buffer containing 25 mM HEPES (ObaG and ObaH)/50 mM Tris-HCl (ObaI) at pH 7.8 containing NaCl (300 mM), MgCl2 (15 mM) and glycerol (10%). ObaG and ObaH buffers were further sup- ple...