Is cytochrome P-450., a transmembrane protein?doi:10.1016/0014-5793(91)80341-yS.A. UsanovA.A. ChernogolovV.I. Chashchin
A BLAST search for the ORFs revealed that the predicted protein encoded members of the cytochrome P450 family. When comparing the sequences of all predicted P450 proteins in D. melanogaster and Anopheles gambiae genomes, the full-length clone of prgv0240 was most similar to CYP306A1 family ...
Cellular IDO, cytochrome b5, and α-tubulin protein were analyzed by Western blotting of cell lysates as described under “Experimental Procedures.” The Western blot shown is a representative of four independent experiments. B, IDO (black columns) and cytochrome b5 (gray columns) protein levels ...
CYTCIP CYTD CYTED CYTET CYTF CYTIP CYTMB CYTO CYTO- Cytochrome C Oxidase Subunit I CYTOL CYTP CYTR CYTS CYTSA CYTT CYU CYUL CYUT CYUY CYV CYVA CYVC CYVD CYVMV CYVO CYVR CYVSA CYVV CYW CYWA CYWC CYWE CYWEN CyWIG ▼
The range of microsomal metabolism from 16 individual liver specimens was 0.13 to 0.99 nmol/min/mg protein. Using 3 microsomal samples exhibiting different maximal velocities, the Km of 1-nitropyrene metabolism was 3.3 +/- 0.5 microM, indicating that perhaps a single or similar cytochromes P-450...
This cDNA encodes a protein of 508 amino acids having a predicted molecular weight of 57,379.82. High-stringency screening of a human testicular cDNA library yielded a partial clone containing 1303 identical bases. RNA gel blots and nuclease S1-protection experiments confirm that the adrenal and ...
cytochrome b5; CYP3A4, cytochrome P450 3A4; CPR, NADPH-cytochrome P-450 reductase; ER, endoplasmic reticulum Abstract Cytochrome P450 3A4 (CYP3A4), is the dominant human liver hemoprotein enzyme localized in the endoplasmic reticulum (ER), and is responsible for the metabolism of more than ...
Heterotrimeric G proteins are known to function as messengers in numerous signal transduction pathways. The null mutation of RGA (rice heterotrimeric G protein α subunit), which encodes the α subunit of heterotrimeric G protein in rice, causes severe d
, 1980) as a marker of mitochondrial membrane potential (Chen, 1988) and of cytochrome oxidase activity (Wharton and Tzagaloff, 1967). RESULTS Assessment of the Purity of Mitochondrial Preparations Electron microscopy examination did not demonstrate the occurrence of extramitochondrial components in ...
We conclude that the cytochrome P450 enzyme CYP86A22 is the key fatty acyl-CoA ω-hydroxylase essential for the production of ω-hydroxy fatty acids and the biosynthesis of triacylglycerol-/diacylglycerol-based estolide polyesters in the petunia stigma....