Mutations in mitochondrial DNA (mtDNA) play critical roles in many human diseases. In vivo visualization of cells bearing mtDNA mutations is important for resolving the complexity of these diseases, which remains challenging. Here we develop an integrated nano Cas12a sensor (InCasor) and show its ...
Fluorophores were excited using a 405 nm diode laser (Hoechst), 488 nm Argon laser (GFP, GFP) or 543 nm HeNe laser (Alexa 546). Embryos were imaged whole mount in MatTek dishes (Ashland). Spinning disc confocal 3D timelapse data was acquired using Volocity acquisition software http://www....
averaged 170 nm in hydrodynamic size, and exhibited linear increases in fluorescence intensity (FLI) to micro- and nanomolar concentrations of NO in a sodium nitroprusside (SNP) concentration-dependent manner. In the presence of SNP, the engineered nanosensors demonstrated physical stability at extracell...
An ideal method for understanding spatiotemporal fluctuations in the living cell would cover broad scales, ~1–105 nm spatially and ~1–105 s tempo- rally, which points to the need for label-free methods. In the past decade, quantitative phase imaging (QPI) has 1Quantitative Light ...
Neuroinflammation, characterized by increased reactivity of microglia and astrocytes in the brain, is known to be present at various stages of the Alzheimer’s disease (AD) continuum. However, its presence and relationship with amyloid pathology in cogni
CD47 recognized by its macrophage receptor SIRPα serves as a “don’t eat-me” signal protecting viable cells from phagocytosis. How this is abrogated by apoptosis-induced changes in the plasma membrane, concomitantly with exposure of phosphatidylserine
(Fig.5c). By 8 weeks post-infarction there was a significant decrease in both infarct magnetic susceptibility (Δχ = −0.019 ± 0.034 ppm,P = 1e−4) and total iron concentration (n = 23, [Fe] = 0.06 ± 0.04 mg/g,P < 0.0001). There...
Reactor technology for the 21st Century must develop with characteristics that can now be seen to be important for the future, quite different from the things important when the fundamental materials and design choices for present reactors were made in the 1950s. Argonne National Laboratory, since ...
(NM), pathology, endoscopy, ophthalmology, or many other types of medical images. While this description is directed to viewing and tracking of medical images, the methods and systems described herein may also be used in conjunction with non-medical images, such as, images of circuit boards, ...
Grejner-Brzezinska, Airborne Integrated Mapping System: Positioning Component, Proceedings of the 53rd Annual Meeting of the Institute of Navigation, Albuquerque, NM, Jun. 1997, 225-235. CA Jan. 30, 2015 Response to Examiner's Report issued Oct. 23, 2014 in Canadian Patent Application No....