By concentration rivanola determined hyaluronic nidazy activity. Before administration rivanola the mixture was added Triton X-305 or X-100 at a ratio of 4: 1 and incubated for 5 min. Possible accurate quantification of enzyme content in the sample in a pH range from 2.2 to 8.0 substrate ...
Various activity of papain such as proteolyticactivity, endoesterolytic activity, amidolytic activity also studied. Enzyme-linked ImmunosorbentAssay For Hyaluronidase (HAase) also been studied. Sandwich enzyme immunoassay is for the in vitroquantitative measurement of HAase in human serum, plasma, tissue...
(1992) An ELISA-like assay for hyaluronidase and hyaluronidaseinhibitors. Matrix 12, 397–403. PubMed CAS Google Scholar Frost, G. I. and Stern, R. (1997) A microtiter-based assay for hyaluronidase activity not requiring specialized reagents. Anal. Biochem. 251, 263–269. Article PubMed ...
MethodsHyaluronidaseactivity in 100 serum specimens were determined by ELBA - like assay. 方法采用ELISA -like 方法检测了卵巢恶性肿瘤患者术前血清、术后随访血清中透明质酸酶的活性. 互联网 Hyaluronidaseis an enzyme that degrades and breaks down hyaluronic acid in your skin. ...
hyaluronidase 英[haɪəlʊ'rɒnɪdeɪs] 美[ˌhaɪəlʊ'rɒnɪˌdeɪs] n.透明质酸酶 网络透明质酸酶;透檬酸酶;玻璃酸酶;玻璃糖醛酸酶 双语例句 1 . MethodsHyaluronidaseactivity in 100 serum specimens were determined by ELBA - like assay....
The enzyme activity was studied in the presence and the absence of SDS on two different 10% polyacrylamide gels (0.75 mm slab) with 0.002% HA incorporated. Gel A was mixed using AA assay buffer, pH 5.0, as described above in the dot method. In gel B, the assay buffer was replaced by...
HA-coated plates were incubated with serial dilutions of Streptomyces HA'ase, and the undegraded HA was measured. This established a standard curve for HA'ase activity against which all unknown enzyme samples were compared. The assay is easily modified to also serve a measure of HA'ase ...
The present study attempts to establish the isoforms of hyaluronidase enzyme and their possible role in the spreading of toxins during envenomation. Screening of venoms of 15 snakes belonging to three different families revealed varied hyaluronidase activity in ELISA-like assay, but with relatively simil...
Among ethanol, methanol and water extracts, the ethanol extract dose-dependently inhibited the ovine, mouse testicular and Vipera russelli snake venom hyaluronidase enzyme activities, with IC50 values 12.00 ± 0.45, 49.40 ± 1.58 g and 125.42 ± 2.82 g mL–1, respectively. In a zymogram assay,...
9.In fact, your doctor will be able to dissolve it with an injection of an enzyme called hyaluronidase, which will work its filler-dissolving magic within about 48 hours. 10.Methods Hyaluronidase activity in 100 serum specimens were determined by ELBA - like assay. ...