北京泽平代理的基础研究用Lonza美国进口人骨髓来源间充质干细胞(Human Bone Marrow Mesenchymal Stem Cells,BMSCs或hMSCs,又称为人骨髓间充质细胞),来自正常(非糖尿病)成年人,是从捐赠者的后双侧穿刺取出的骨髓分离而来,传代2次后冷冻保存,建议在第5次传代前使用。每批次细胞确认CD44、CD73、CD90、CD105、CD166...
Human MSC1 and MSC2 mesenchymal stem cell preparations were harvested after written informed consent from the bone marrow of healthy volunteers and isolated as published previously51,52. MSCs were cultured in Mesenchymal Stem Cell Growth Medium (Lonza, Basel, Switzerland) with added MSCGM™ Single ...
Human mesenchymal stem cells (hMSC) are of tremendous interest for the field of regenerative medicine and cell therapy due to their tissue regenerative properties and potential to modulate the immune system. An inherent characteristic of hMSC is their ability to differentiate into chondrocytes, ...
Human mesenchymal stem cells (hMSCs, PT-2501, Lonza) were obtained from randomly selected healthy donors of both sexes with ages ranging from 19 to 38 years old and cultured in dedicated medium MSCBM (PT-3238) supplemented with 10% MCGS (PT-4106E), L-glutamine (PT-4107E), and gentamicin...
Stem Cells. 2010, 28: 564-572. PubMed Central CAS PubMed Google Scholar Ito T, Sawada R, Fujiwara Y, Seyama Y, Tsuchiya T: FGF-2 suppresses cellular senescence of human mesenchymal stem cells by down-regulation of TGF-beta2. Biochem Biophys Res Commun. 2007, 359: 108-114. 10.1016/...
After enzymatic digestion of liver tissue, single ce ll suspensions were seeded into plastic flasks at the density of 7 脳 105 cells/cm2 in a MSC-specific medium (MSCGM, Lonza, Italy). HSc were isolated by standard gradient separation. Cell characterization was performed on both cuboidal and ...
Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon...
Peripheral blood mononuclear cells (PBMCs) were isolated from the blood of healthy donors collected in heparin tubes. We diluted whole blood 1:1 with Roswell Park Memorial Institute (RPMI) medium (Lonza, Basilea, Sweeden) and layered 2 ml on top of 4 ml of Ficoll solution (Alere Technologies...
gene, 80494) containing sequences for an AAVS1 locus-specific single guide RNA (GGG GCC ACT AGG GAC AGG AT) and the Cas9 nuclease and 3 µg of donor construct (pAAVS1-CAG-FRT-flanked STOP-mKate2-HA-poly(A)) following the Lonza Amaxa 4D Nucleofector protocol for human stem cells...
hPSCs were then electroporated in a 16-well Nucleocuvette Strip, using the 4D Nucleofector system (Lonza) with the CA137 electroporation code38. Following electroporation, cells were plated into one well of a Matrigel-coated 24-well plate containing 500 μL of mTeSR1 media supplemented with ...