BioLegend product webpage product information Apps. Abbrev. : FC, ICC, ICFC Cat # : 422301 Item : Human TruStain FcX™ Other Names : Fc Blocker, FcγR blocker, Fc inhibitor Size : 50 tests Price (USD) : 101 USD Conjugate/Tag/Label : ...
Both fractions were resuspended in Fc buffer (HF + 1:1000 human Fc block (BD Biosciences, Missisauga, ON, Canada)) to a final concentration of 0.5 M cells/mL. CD45-PE antibody (Biolegend) was added at 1:200 and the cell suspension was incubated for 30min on ice in the dark. After...
人Fc受体阻断剂(Fc Receptor Blocking Solution, Human)特别开发用于阻断FcR引起的非特异性染色,且不会干扰抗体介导的人细胞特异性染色。本品与抗人CD16(克隆3G8),CD32(克隆FUN-2)和CD64(克隆10.1)抗体的流式染色兼容。 产品特性 别名 Fc Blocker,FcR blocking reagent,FcγR blocker,Fc inhibitor ...
respectively. Huh7.5 cells and NPCs were infected with ZIKV for 24 h at an MOI of 1. The cells were then collected, incubated with Zombie Violet™ Fixable Viability stain (BioLegend) for 20 min at 4 °C, washed, fixed with 4% paraformaldehyde, and permeabilized with 0.1% saponin...
APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC Lymphopure™ Cell Sep - Pos,Cell Sep - Neg True-Stain Monocyte Blocker™ FC Compensation Beads FC Tandem Stabilizer FC Related Pages & Pathways Pathways Innate Immunity Stem Cells and Development Pages Quality Control Multicolor ...
Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laborat...
Primary human macrophage cultures were established by treating peripheral blood monocytes with 50 ng/ml of M-CSF (Biolegend) in RPMI1640 complete media for 7 days. Naïve macrophages (Mo) so obtained were fur- ther stimulated with 50 ng/ml of LPS and 10 ng/ml of human IFNγ...
(4 parts Cell Staining Buffer + 1 part True-Stain Monocyte Blocker [BioLegend, San Diego, CA, Cat# 426103) (Table1)]. The samples were each stained with 25 µL of pre-made stain index optimized anti-human CD3-PE/Cy7 antibody (BioLegend, San Diego, CA, Cat# 317334) (Table1...
Cell suspension was washed with ice-cold FACS buffer (2% FCS and 0.1% NaN3 in PBS) and incubated with Fcγ blocker (BD Biosciences Pharmingen, 1 µg/ml) for 30 min. Cells were then washed in ice-cold FACS buffer and stained with PE/Cy7 anti-human CD31 antibody (BioLegend, ...
PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC Lymphopure™ Cell Sep - Pos,Cell Sep - Neg True-Stain Monocyte Blocker™ FC Compensation Beads FC Tandem Stabilizer FC Related Pages & Pathways Pathways B cell Development and Activation B-Cell Receptor Pathway CD28 Signaling in...