For gel shift assays, 5 µg of the nuclear proteins was incubated with 2 µL of 5× gel shift binding buffer) Promega, Heidelberg, Germany) and 2 µL of γ-P32-labeled probe (for Supershift assays, 2 µL of the antibody was added), filled up to a volume of 10 µL with...
5 µg of the nuclear proteins was incubated with 2 µL of 5× gel shift binding buffer) Promega, Heidelberg, Germany) and 2 µL of γ-P32-labeled probe (for Supershift assays, 2 µL of the antibody was added), filled up to a volume of 10 µL with H2O and incubated for...