本发明属于分析检测技术领域,具体涉及一种基于纸基底的自动化HPLCSERS联用装置和检测方法.该装置包括高效液相色谱仪,拉曼光谱仪和接口,所述接口包括纸基底和卷纸器;所述纸基底包括疏水滤纸和均匀间隔附着在所述疏水滤纸表面的SERS基底点,所述SERS基底点包含贵金属纳米粒子;所述高效液相色谱仪的样品流出端口设有管道,...
德国的一项研究表明,表面增强拉曼光谱(SERS)在食物中类胡萝卜素和其他微量营养素研究方面是一个比HPLC更加可靠的工具。 抗氧化剂对人体健康的确切益处目前正在讨论中,特别是膳食补充剂,它目前仍然是我们吃的水果和蔬菜中似乎对生理指标有积极的影响彩色色素。在番茄中发现的红色的番茄红素就是一种这样的化合物,它一般...
表面增强拉曼光谱、高效液相色谱、联用技术、邻氨基苯硫酚、邻碘苯甲酰氯通过表面增强拉曼光谱(SERS)与高效液相色谱(HPLC)联用开展了在线监测催化剂对邻氨基苯硫酚(OATP)与邻碘苯甲酰氯反应的影响.结果表明,无催化剂时反应产物为1b{S-[2-(2碘苯甲酰氨基)苯基]-2-碘代苯硫酸盐}和2b{N,N′-[二硫(2, 1-...
Due to the disposable nature of the SERS substrate, the new method does not suffer from the known "memory effect" which is known to lead to false positive identification in traditional HPLC-SERS methods. Therefore, the presented HPLC-paper SERS platform holds great potential for the sensitive ...
The quantification limit of PSI-6206 by SERS was found to be 13 ng L1 (R2 value = 0.959, RSD = 5.23%). For the structural and quantitative analysis of PSI-6206 in blood plasma, an interference-free HPLC-SERS method was developed and compared to HPLC-DAD and HPLC–MS methods. The ...
KeywordsAloe veraFORSHPLC-DAD-MSRed safflowerSERSTextileAbbreviationsAgNPAg nanoparticlesFAMWformic acid/methanol/water extractionFOMFFibre Optic Molecular FluorimetryFORSFibre Optic Reflectance SpectrophotometryTICTotal Ion CurrentXICeXtracted Ion Current. Intensity of Raman bands(w)weak(mw)medium weak(m)...
After extraction, Ag colloid pastes were applied on the gels for SERS analyses, allowing for the identification of the three dyes. For the HPLC-MS/MS analyses, re-extraction of the dyes was followed by a clean-up step that was successfully applied on madder and turmeric. The colour change ...