Mutagenesis is commonly used to validate cryoEM structures. Given the extensive number of published mutants, designing new mutations was unnecessary. From this, we identified many assembly-deficient mutations30,32,43 that affect residues that form strong interactions at subunit interfaces (Extended Data...
We thus performed two sets of new studies to further validate the class I and II models, respectively, using mutations located away from the TCR and pMHC binding interface but capable of impacting their respective conformational changes under force, which were analyzed by MD simulations, bond life...
A theoretical first step in drug discovery is to identify and validate specific molecular targets. With the expanding knowledge of microbial biochemistry and genetics, it has now become possible to identify important microbial enzymes, receptors and processes at the molecular level that might be suitabl...
To further characterize and validate the specificity of the antibodies, we assessed their binding affinity and kinetics to monomeric and oligomeric α-syn species using SPR (Fig. 7A). The antibodies were immobilized on the SPR chip surface at low densities. Fig. 7 and Supplementary Fig. 4 show...
PipeIT2, with its performance, reproducible results, and user-friendly execution, significantly enhances molecular diagnostic laboratories. Disease outbreaks and stress in fish farms utilizing tanks and sea cages for intensive fish rearing are directly correlated with impaired growth, reproduction, and meta...
epistasis modelling methods that are easier to interpret. Standard operating procedures are underway to replicate and validate GWAI results, along with appropriate definitions for these. We need better experimental methods for confirming statistical models of epistasis in animal models or in human cell ...
Once your cluster is up and running you will want to validate it by running some benchmarks and sample applications. There are various benchmarks and code samples for GPUs and the network as well as applications to run on the entire cluster. For GPUs, you need to run two basic tests. ...
epistasis modelling methods that are easier to interpret. Standard operating procedures are underway to replicate and validate GWAI results, along with appropriate definitions for these. We need better experimental methods for confirming statistical models of epistasis in animal models or in human cell ...
Fourier Shell Correlations performed by 3DFSC66, Mtriage71, and Resmap72 were used to validate the final resolution. The number of particles included in the analysis and the strategy utilized are summarized in Supplementary Table 1. Model fitting and model-to-map cross correlation fit were ...
To validate this further, we have performed the similar analysis on the control system, i.e. the CE-bound CETP crystal structure. As Fig. S4a,b show, the protein maintained its structural stability in both complexes with equal propensity. More specifically, the persistence of α-helices and ...