f, Heatmap of the TS intensity in n = 143 cells (rows) in the presence of RSC. Yellow: fluorescence intensity of TS; blue: region excluded from analysis. g, Cells that were depleted of RSC (red) showed an increased GAL10 induction time compared to the nondepleted cells (gray). ...
Viral evolution experiments were conducted by using a previously characterized HPIV3 neutralizing monoclonal antibody PIA174Ab29to select for variants that are infectious in the presence of the antibody. Two EV viruses emerged (Fig.1A, B). One that we previously characterized functionally (EV-1) be...
How HJURP works is still a mystery. It has been recently speculated that its action at centromeres could be coupled to DNA repair [23] or to transcription [24]. In vitro, Scm3 can incorporate CENP-A-containing octamers into plasmid DNA, but not octamers bearing canonical H3 [25]. This...
DNA is the genetic material of all living organisms, which carry hereditary units, genes from parents to the daughter cells. The DNA is present in the nucleus of eukaryotes or the nucleoid of prokaryotes. Fredrich Miescher discovered the DNA in 1860, but scientist Watson and Crick also played...
Additionally, double gene mutant citrus plants have been developed using a single plasmid containing CBE- and Cas12a-mediated editors targeting theCsALSandCsLOB1genes, respectively (Huang et al.2023a; Jia et al.2024). Application of gene editing technology incitrusresearch ...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
How the DNA structure forms the genetic code, and how that genetic code comes to be expressed as a phenotype?Central Dogma of Molecular Biology:In biology, the central dogma refers to protein synthesis on the basis of gene sequences in DNA. A gene ...
However, because lncRNAs can function in cis, it's important that there is the significant mechanistic characterization of the candidate lncRNA to justify its cloning into a plasmid. The same catalytically inactivated Cas9 has also been fused to a transcriptional activation domain, for example, the...
Studies showing only a 34-mer suggest that other stoichiometries arise from artefacts due to C-terminal proteolysis of FliF or incorrect templating during plasmid expression9. We can exclude C-terminal proteolysis affecting stoichiometry in our structure because we observe density for the full C-term...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l