A negative control was included, containing all components except mycelium. The microtubes were placed in a macerator at 4000 rpm for 90 s, followed by incubation in a dry bath at 65 °C and 500 rpm for 30 min. After incubation, 200 µL of Protein Precipitation Solution was added to ...
of a main effect of treatment being identified in the ANOVA. These results demonstrate for the first time that the performance enhancing effects of caffeine may not be repeatable between days, where our data uniquely indicates that this is in part attributable to between sessions variation in ...