本文采用RT PCR技术从人外周血PBMC中克隆HLA A2基因的胞外区 ,拼接上依赖BirA酶的可生物素化序列后 ,装入pET 42b (+)高效表达载体进行诱导表达。并对表达产物进行纯化与复性。结果成功地扩增出HLA A2基因并测序证实 ;构建了融合表达载体pET HLA A2 ,并获表达与纯化。为研究在原核系统中表达、纯化与复性及进一步...
以HLA A2 + 健康人和慢性乙型肝炎患者的PBMC为实验对象 ,进行体外诱导CD8+ T细胞应答的免疫学功能研究.结果 所设计治疗性多肽分子可在体外诱导较强的抗原特异性CD8+ CTL应答 ;" AAA "铰链区设计和"Th +B"细胞表位的引入可增强CTL表位肽的免疫原性.结论 提示在治疗性表位多肽疫苗的分子设计中 ,短而高柔性的"...
目的:探讨体外激发HLA—A2型外周血单个核细胞(PBMC)中的MAGE-A10(254-262)特异性T淋巴细胞及其功能检测方法.方法:分别采用肽段MAGE—A-10(254-262)直接刺激PBMC的Bulk Culture法和自体DC负载肽段MAGEA10(254-262)刺激CD3^+CD8^+法,均经3次刺激进行体外扩增tetramer—MAGE—A10(254—262)阳性T细胞.以人流感病毒...
结果:HLA-A2型PBMC用BuckCulture或DC负载肽段刺激CD3+CD8+法培养,激发的tetramer-flu阳性T细胞率均>10%,DC法激发的tetramer-flu阳性T细胞率显著高于Buck Culture,t=9.79,P<0.01。Bulk Culture法得到的阳性T细胞率分别为0.01%和0.02%,DC法则分别是0.05%和0.12%。Sorting得到的tetram-er-MAGE-A10(254-262)阳性...
PBMCs)为效应细胞、同时表达肝素酶和 HLA.A2的人体HCC.LM6肝癌细胞和SW-480结肠癌细胞为靶细胞,效:靶为40:1 时Hpa310-3ls(FLNPDVLDI)刺激效应细胞对HCC.LM6肝癌细胞的溶破率为52.48%, 对SW-480结肠癌细胞的溶破率为44.17%,而其他各条候选肽没有明显的特异性溶 ...
本文采用RT PCR技术从人外周血PBMC中克隆HLA A2基因的胞外区 ,拼接上依赖BirA酶的可生物素化序列后 ,装入pET 42b (+)高效表达载体进行诱导表达。并对表达产物进行纯化与复性。结果成功地扩增出HLA A2基因并测序证实 ;构建了融合表达载体pET HLA A2 ,并获表达与纯化。为研究在原核系统中表达、纯化与复性及进一步...
五.腺病毒转染PBMC36小时后,分别作T2细胞负载Sur79M2(HLA-A2+,Survivin+),肝癌细胞株HepG2(HLA-A2+,Survivin+),乳腺癌细胞株Mcf-7(HLA-A2+,Survivin+),肝癌细胞株7402(HLA-A2-,Survivin+)及肺癌细胞株NCI-H1299(HLA-A2-,Survivin-),12h,24h,36h三个时间点MTT检测杀伤效果及Elisa法检测IFN-γ的分泌. ...
Peptide-specific CTL-precursors reactive to 16 kinds of vaccine candidates in the pre-vaccination peripheral blood mononuclear cells (PBMCs) were measured, and patients were followed by vaccination with only positive peptides (up to 4 kinds of peptides). Serum prostate-specific antigen (PSA) ...
HLA-A2 PBMCs (Catalog #70048) are selected for HLA-A2 positive donors and include the same characterization criteria as Standard PBMCs and Characterized PBMCs. Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at...