donorto specificallylyseHCC・ LM6cellsandSW-480cellswhich expressing both Hpa and HLA—A2 protein. Theseresultsshowedthatthe nona-peptidenpa310-318(FLNPDVLDI)we fredfirst may beanovelidentifiedHLA-A2一restrictedCTL epitope,which is capable
Direct cloning of leukemia-reactive T cells from patients treated with donor lymphocyte infusion shows a relative dominance of hematopoiesis-restricted minor histocompatibility antigen HA-1 and HA-2 specific T cells. Leukemia 2004; 18: 798–808. Article CAS PubMed Google Scholar Bodinier M, ...
Our study also showed that comprehensive DNA matching for A2 would eliminate most A mismatches in the unrelated-donor transplantation in study populations.doi:10.1016/0198-8859(96)84712-4Bronwyn MathesonXiaojiang GaoelsevierHuman Immunology
Indirect allorecognition is the stimulation of recipient T lymphocytes by donor-MHC processed to peptide and presented in the context of self-MHC. In experimental models of transplantation, indirect allorecognition may contribute to acute or chronic rejection (2, 3, 4) and is necessary for the ...
HLA-DPB1 and DPA1~DPB1 linkage mismatch affects the survival of recipients receiving HLA-14/14 matched unrelated donor HSCT To analyse the effect of HLA-DPA1 and HLA-DPB1 allelic mismatches on the outcomes of unrelated donor haematopoietic stem...
The assays based on Luminex technology are currently the most popular to detect anti-HLA antibodies, due to their high sensitivity and specificity, such as LABScreen Single Antigen (SA One Lambda, Canoga Park, CA) and Donor Specific Antibody (xmDSA) (DSA Tepnel Lifecodes, Stamford, CT). ...
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in you...
The fact that we could detect such T cells in only one donor suggests that the frequency of these T cells is very low or that they are naive T cells. In order to improve the sensitivity of ELISPOT assay, we tried repeated in vitro stimulation of PBMC in cell culture with different ...
Donor PBMC were plated at a density of 107cells/ml together with 5 × 104/ml HPV-16 peptide-loaded irradiated antigen-presenting cells in 24-well culture plates in DMEM medium supplemented with 10% human serum, 1% l-glutamine, 1% penicillin/streptomycin, 0.05 mm 2-mercaptoethanol, and 10 ...
Ethical considerations on preimplantation genetic diagnosis for HLA typing to match a future child as a donor of haematopoietic stem cells to a sibling Recently, several requests were made by couples with an affected child who wanted preimplantation genetic diagnosis (PGD) to select embryos in the ...