HIFBackground: To explore the roles of HIF1 alpha- and HIF2 alpha-regulated BNIP3 in hypoxia-induced injury of neurons.Zhu, LeiQi, BoxiangHou, DaorongXuzhou Med Univ Xuzhou Childrens Hosp Dept Intens Care Unit Xuzhou 227006 Jiangsu Peoples RPathology Research and Practice...
Hypoxia induces the expression of the pro-apoptotic gene BNIP3 It has been shown that oxygen deprivation results in apoptotic cell death, and that hypoxia inducible factor 1 (HIF1) and the tumor suppressor p53 play key... K Guo,G Searfoss,D Krolikowski,... - 《Cell Death & Differentiatio...
As acidosis is known to activate BNIP3-mediated death, we examined other secondary stresses, such as oxidants or prolyl hydroxylase activity are necessary for exposing the prodeath functions of HIF in neurons. Antioxidants or prolyl hydroxylase inhibition prevented potentiation of death by HIF-1alpha...
heterodimers engagep300/CBPin the nucleus for binding to hypoxic response elements (HREs), inducing transcription, and thus regulation of genes (e.g.,EPO,VEGFA). HIF-1 predominantly transactivates genes involved in glycolytic control and pro-apoptoticgenes (e.g.,LDHAandBNIP3), and HIF-2 ...
Activated HIF1a also induced BNIP3 and BNIP3L expression, markers for the autophagic destruction of mitochondria. Most importantly, fibroblasts expressing activated HIF1a increased tumor mass by ~2-fold and tumor volume by ~3-fold, without a significant increase in tumor angiogenesis. In this ...
Ryan, Growth factor signaling permits hypoxia-induced autophagy by a HIF1alpha-dependent, BNIP3/3L-independent transcriptional program in human cancer cells, Autophagy 5 (2009) 1068e1069.Wilkinson, S., and Ryan, K.M. (2009). Growth factor signaling permits hypoxia-induced autophagy by a HIF1...
exponentially growing cells via hypoxic stimulation is independent of the Akt/mTOR pathway whereas HIF-1 activation obtained in high confluency is totally dependent on mTOR pathway as rapamycin totally impaired (i) HIF-1 stabilization and (ii) mRNA levels of CA9 and BNIP3, two HIF-target genes...
In conclusion, 2ME2 and D609 are powerful agents to protect brain from cerebral ischemic injury by inhibiting HIF-1alpha expression, attenuating the superfluous expression of VEGF to avoid blood-brain barrier disruption and suppressing neuronal apoptosis via BNIP3 pathway....
Assessment of mRNA levels of three Hif-1alpha target hypoxic response element (HRE) genes, Glucose transporter -- 1 (GLUT-1), Vascular endothelial growth factor a (VEGFa), and BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), as well as measurement of luciferase activity ...
Furthermore, HX increased HIF-1&agr; activation, which was enhanced by cotreatment with EL and resulted in transcription of the cell death proteins Nix and BNIP3. The transactivation of HIF-1alpha was mediated in part by p38 MAPK signaling in HX/EL-cotreatment.;The results indicated that ...