(b) Representative photomicrographs of hematoxylin-eosin (upper panel) and oil red O staining (lower panel) of mice liver sections (n ¼ 4 per group); (c) total liver TG content, serum AST, ALT and TG levels (n ¼ 9 AAV8-GFP and 10 AAV8-TAp63a); (d) protein levels of FAS...
Biomarkers of fibrosis are commonly divided into Direct and Indirect markers. Direct markers are fragments of the liver matrix components produced by hepatic stellate cells (HSC) during the process of ECM remodeling. Indirect markers include molecules released into the blood due to liver inflammation, ...
Basal blood glucose levels were measured after an overnight fast (12 h) with an Accucheck glucometer (Roche). GTT and ITT were done after an intraperitoneal injection of either 2 g kg−1D-glucose (Sigma-Aldrich, USA) or 0.75 U kg−1insulin (Sigma-Aldrich, USA) and area...
Samples were probed for sortilin (upper panel) and apoB100 (lower panel) (B, right). Coimmunoprecipitation of endogenous apoB from human liver with anti-sortilin IgG (lane 3) but not with nonimmune control IgG (lane 2). As a positive control, immunoprecipitation of apoB100 with anti-ap...