Recovery of the TMV required more time in S-HEK cells: the percentage of experiments where the signal did not reach 50% or 90% recovery towards the baseline in 2.5 s after pulse exposure (during the observation time) was higher in S-HEK cells than in NS-HEK cells (Fig.3c,d). Time ...
(red), sarcomeric actinin (Sarc.Act, green) and nuclei (DAPI, blue) suggesting robust NaV1.5 expression for WT, MUT1 and MUT2 cells, confirmed by a lack of SCN5A staining when adding the NaV1.5 block peptide. (f) Confocal images for IF staining of plated hiPSC-CMs (WT and the two...
Western blot analysis HEK293 and HEK293-APPswe cells treated with L655,240 or vehicle for 24 h were harvested in 2×sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS–PAGE) sample buf- fer (25% SDS, 62.5 mmol/L Tris-HCl, pH 6.8, 25% glycerol, and 0.1% bromophenol blue)...