A hepatitis C virus (HCV) plasmid vaccine was constructed, based on class II-associated invariant chain peptide (CLIP) substitution which endogenously targets HCV non-structure protein 3 (NS3) CD4+ T helper 1(Th1) epitope (1248AA-1261AA) to major histocompatibility complex (MHC) class II antig...
François-Loïc Cosset Part of the book series:Methods in Molecular Biology™((MIMB,volume 510)) 2756Accesses Abstract HCV infection leads in 50 to 80% of cases to chronic hepatitis, liver cirrhosis, or hepatocellular carcinoma. Interferons and the nucleoside analog ribavirin form the basis ...
Competitive binding assays were performed in triplicate on 384-well plates (#3575, Corning) with a total well volume of 15 μL. Each well solution contains FAM-labelled HIF-1α peptide JC9 (10 nM)82, VCB protein (15 nM), and decreasing concentrations of Cyp-PROTAC or Cyp-PROT...
Part of the book series: Methods in Molecular Biology ((MIMB,volume 1911)) 2374 Accesses Abstract For a long time, the study of the HCV infectious cycle has been a major challenge for researchers because of the difficulties in generating an efficient cell culture system leading to a producti...
储备液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C储存时,请在2年内使用, -20°C储存时,请在1年内使用。 摩尔计算器 稀释计算器 Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) 质量 ...
P670. Autoimmune hepatitis type 2 and HCV infection: Study of HLA-class II antigensautoimmune hepatitis type 2 and hcv infection: study of hla-class ii antigens.doi:10.1016/0198-8859(96)85365-1A JuradoB CárdabaP JaraL HierroP Palomino...
Microwave irradiation of contaminated drug preparation cigarette filters with HCV and/or HIV-1. Slim cigarette filters, 6 mm × 15 mm (Gizeh, Gummersbach, Germany), were soaked in reporter HCV (Jc1) or HIV-1 containing culture fluids on the spoon with a volume of 200 ...
for 3 minutes, and then mixed with equivalent volume of cyanin-probe and hybridization solution (6×SSC, 0.5% SDS) respectively. [0037] 3) With chambers covered, 50-200 ul of hybridization buffer was filled in chambers on the slide, and then reacted at 63° C. for 1-3 hours. [...
is 1012virions per day. HCV particles in the serum exist as a mixture of complete low-density infectious lipo-viral particles (LVP) and a vast excess of apoB-associated empty nucleocapsid-free sub-viral particles that are complexed with anti-HCV envelope antibodies. Apolipoprotein E (apoE) is...
MHC class I and II predictions were determined using the Immune Epitope Database (IEDB) (http://tools.immuneepitope.org/main/). For prediction of CTL epitopes, “ctlpred” and ANN methods were used (48). Probability of antigenicity was expected by VaxiJen software athttp://www.ddg-pharmfac...