put into a recording chamber on the stage of an inverted microscope (Nikon Diaphot, Melville, New York, NY, USA) and superfused with Tyrode's solution (36 ± 0.2 °C) containing (mM): NaCl 140, KCl 5.4, CaCl2 1.8, MgCl2 1.0, glucose 5.5, HEPES 5.0; pH 7.4 (adjusted with NaOH)....
The bath solution for whole cell recording was normal Tyrode solution containing (in mM) 140 NaCl, 5.4 KCl, 1.8 CaCl2, 0.5 MgCl2, 0.33 NaH2PO4, 5.5 glucose, and 5.0 HEPES (pH was adjusted to 7.4 with NaOH). The pipette solution contained (in mM) 70 potassium aspartate, 50 KCl, 10 K...