h process h slag h somewhere in time hangen von d h-k-atpase inhibitors hcfh human complement h- homo h-e h-health h-j international tia h-nsp h-o theory h-rion process h harmonious society h albicilla h cepranensis h davidii franch h debile hemsl c y wu h georgicus h l m ...
Escherichia coli H(+)-ATPase: role of the delta subunit in binding Fl to the Fo sector The roles of the Escherichia coli H(+)-ATPase (FoFl) delta subunit (177 amino acid residues) was studied by analyzing mutants. The membranes of nonsense (Gln-23---end, Gln-29---end, Gln-74--...
Dicyclohexylcarbodiimide, which inhibits proton translocation in the vacuolar ATPase, predominantly labeled two polypeptides of 18 and 15 kDa, which compose the membrane sector of the enzyme. These results suggest that the lysosomal H+-ATPase is a multimeric enzyme, whose subunit structure is similar ...
The assembly of the yeast vacuolar H(+)-ATPase requires the attachment of several cytoplasmically oriented, peripheral subunits (the V1 sector) to a complex of integral membrane subunits (the Vo sector) and thus is not easily described by the established mechanisms for transport of soluble or ...
Assembly of peripheral subunits of the vacuolar H+-ATPase. The V1 sector is shown as being assembled from the individual peripheral subunits prior to attachment to the integral membrane Vo sector. In the mutant cells examined, the tfplΔ and vma4Δ cells appear to be arrested at the first ...
Negrin, R. S., Foster, D. L., and Fillingame, R. H., 1980, Energy transducing H+-ATPase ofEscherichia coli: Reconstitution of proton translocation activity of the intrinsic membrane sector,J. Biol. Chem.255:5643–5648. PubMedCASGoogle Scholar ...
The main determinant of acidic pH at the Golgi is the vacuolar-type H(+)-translocating ATPase (V-ATPase), whose V1domain subunitsBandCbind actin. We have generated a GFP-tagged subunitB2construct (GFP-B2) that is incorporated into the V1domain, which in turn is coupled to the V0sector...
Inhibition of enzyme activity is usually coupled with removal of the V1 sector from the membrane (11, 16, 17, 18, 19, 20). Both inhibition and subunit stripping appear to be favored by the presence of ATP. For the yeast vacuolar H+-ATPase, loss of the V1 subunits is greatest in ...
The V-ATPase is a proton pump that creates an acidic medium, necessary for lysosome function and vesicular traffic. It is also essential for several developmental processes. Many enzymes, like the V-ATPase, are assemblies of multiple subunits, in which e
拟南芥V-ATPase由13个亚基组成,包括V。和V。两个结构域。c亚基是、k域的 重要组成部分之一,现已踞确由五个同源基因编码,以6拷贝的形式组成V-ATPase 离子进出的通道,是植物响应逆境胁迫中最为敏感的亚基。研究拟南芥V-ATPase C 亚基基因功能特别是其在植物抗非生物胁迫中的作用机理,具有重大的理论意义和实 ...