for a reliable annotation of whole proteomes./p pResults/p pHere we present PredGPI, a prediction method that, by coupling a Hidden Markov Model (HMM) and a Support Vector Machine (SVM), is able to efficiently predict both the presence of the GPI-anchor and the position of the 蠅-site...
BIG-PI is the first publicly released method for GPI-anchor prediction, and the predictions are made by four kingdom-specific predictors [10,12,13]. This method is able to recognize only half of the submitted GPI-anchored proteins while maintaining a false positive rate as low as 0.3%. Mor...
for a reliable annotation of whole proteomes./p pResults/p pHere we present PredGPI, a prediction method that, by coupling a Hidden Markov Model (HMM) and a Support Vector Machine (SVM), is able to efficiently predict both the presence of the GPI-anchor and the position of the ω-site...
However more accurate methods are needed for a reliableannotation of whole proteomes.Results: Here we present PredGPI, a prediction method that, by coupling a Hidden Markov Model(HMM) and a Support Vector Machine (SVM), is able to efficiently predict both the presence of theGPI-anchor and ...
determines the signal composition of the GPI anchor region, while the SVM takes into consideration the characteristics of the N-terminus of the target protein which make PredGPI independent from SignalP. Among all these programs, PredGPI is the most widely used tool for the prediction of GPI-...
GPI anchor biosynthesis is a complex multi-step process that takes place at the endoplasmic reticulum and involves a multitude of enzymes12. To identify the cause of the GPI anchor defect in BLaER1 cells, we performed a 3′ mRNA sequencing (3′ mRNA-seq) of undifferentiated BLaER1 cells from...
(Supplementary Fig.2). While most GPI-APs depend on GPI-anchoring for surface display, and FACS signals are lost with an incompetent ω-site, proULBP2 is known to anchor to the cell surface via its CSP without GPI-anchoring30, as also demonstrated by the high FACS signal of proULBP2-...
GPI lipid anchor attachmentpost-translational modificationGPI modification predictionfungigenome annotationThe fungal transamidase complex that executes glycosyl... B Eisenhaber,G Schneider,M Wildpaner,... - 《Journal of Molecular Biology》 被引量: 501发表: 2004年 ...
The completed GPI anchor is attached to proteins that have been translocated across the ER membrane and that display a GPI signal anchor sequence at the C terminus. GPI proteins transit the secretory pathway to the cell surface; in yeast, many become covalently attached to the cell wall. Genes...
GPI anchor addition is important for JAGGER localization and in vivo function. Loss of correct GPI anchor addition in JAGGER, negatively affects its locali