BECOME FAMILIAR WITH THE FOLLOWING INSTRUCTIONS BEFORE AN EMERGENCY ARISES. DO NOT USE THIS KIT AFTER DATE STAMPED ON THE VIAL OF GLUCAGON. IF YOU HAVE QUESTIONS CONCERNING THE USE OF THIS PRODUCT, CONSULT A DOCTOR, NURSE OR PHARMACIST. Make sure that your relatives or close friends know that...
- If there has been no response after 15 minutes, an additional 0.5 mg dose (0.5 mL) of GlucaGen® may be administered using a new kit while waiting for emergency assistance. Important Administration Instructions for Using the GlucaGen® HypoKit® to Treat Severe Hypoglycemia (2.1) ...
The Alkaline Phosphatase Conjugate Substrate Kit (BioRad, CA., USA) was used according to the manufacturer’s instructions. Cleavage of the fusion protein The cleavage reaction for the fusion protein was performed using the enzyme enterokinase EKMax (Invitrogen, USA) at a concentration of 1 U ...
The resulting PCR-products were subcloned into the pCRII vector using the TA Cloning kit (Thermo Fisher Scientific) according to the manufacturors instructions. In total 76 clones were analysed by restriction enzyme digestion and sequencing. Single cell RT-PCR Single cell RT-PCR was performed as ...
Abundance of labeled cell nuclei with the protein marker Ki67 in paraffin-embedded tissue sections from jejunum, ileum, and colon was measured as a proxy for cell proliferation following the manufacturer’s instructions (Vector Labs, Burlingame, CA). After immunostaining, the abundance of Ki-67 was...
Then, TRAP staining and TRAP activity were detected using a TRAP-staining kit (Sigma-Aldrich, St Louis, USA) and acid phosphatase assay kit (Beyotime, Shanghai, China) according to the manufacturers’ instructions. 2.5. Bone resorption assay The bone resorption experiment was conducted as ...
In another set, sections were labeled by TUNEL according to manufacturer’s instructions followed by staining with rat anti-insulin antibody. All images were acquired through a fluorescence microscope equipped with a charge-coupled device camera (Olympus Inc. JPN). The ratio of insulin positive beta...
The cAMP signal of GLP-1R/NlucP-293 cells was determined using the Nano-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions. The EC50 values (50% maximal effective concentration) were calculated using GraphPad Prism software version 5.0 (La Jolla, CA, USA). Cell...
Total mRNA was extracted from untreated primary mouse hepatocytes in six-well plates using 1 ml per well of Trizol (Life Technologies) reagent, according to the manufacturer’s instructions. RNA was quantified by Nanodrop, and 500 ng was used for cDNA synthesis using the M-MLV RT kit (...
turer s instructions(Tiangen, Bei ing, China). The content of rhGLP 1 analogue fusion protein was determined according to the modified procedure described in literature [4]. The inoculum of SG2 was transferred to the fresh YPD in a volume ratio of 1:1 (inoculum:YPD). After 24 h...