GFP is an endogenous protein from the jellyfish Aequorea Victoria. It was isolated by Osamu Shimomura in 1962. In 1992, the sequence of GFP was cloned (Douglas Prasher) and Martin Chalfie’s lab expressed the sequence in vivo. Roger Tsien’s lab improved GFP and managed to convert it into...
Protein sequences have evolved to fold quickly and efficiently to their native, active conformation in the complex crowded environment of the cell. A role for co-translational folding in efficient protein folding has been proposed. Yet, the mechanisms by which co-translational folding might affect ...
NCBI 搜protein sequence,然后tblastn去除merge之后的冗余:Remove Duplicates from a Fasta File and manipulate names上次的最佳匹配是这个序列:1 2 >AB971579.1 Synthetic construct sfgfp gene for superfolder green fluorescent protein, complete cds ATGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCG...
The fusion protein is designated CTS del GFP:PR. The del symbol indicates the linkage peptide sequence leu(ile)-N-glu that is cleaved by the T4 head morphogenetic proteinase gp21 during head maturation. The fusion protein is fluorescent and has protease activity as detected by the appearance of...
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. 蛋白标签: N-terminal 6xHis-SUMO-tagged 产品提供形式: Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you ...
Hence, to assess the subcellular localization and function of HIV-1 Vpr, we constructed both N- and C-terminal EGFP or EYFP fusion proteins with Vpr and also used Vpr and/or a His-tagged protein as controls. Subcellular localizations were determined in three cell lines by confocal microscopy,...
DNAvaccineagainstHIV.Methods:Accordingtothepublishedgreenfluorescentproteingenesequencein Genbank,apairofprimerswererespectivelydesignedandsynthesized.Afteramplificationwithpolymerasechain reaction,theproductwasclonedintopMD一18TvectorusingTAcloningfollowedbyBamHIandXhoIdigestion ...
N.C. Shaneret al., A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods. 2013 May;10(5):407-9. doi: 10.1038/nmeth.2413. Epub 2013 Mar 24. Hostettleret al., The Bright Fluorescent Protein mNeonGreen Facilitates Protein Expression Analysis In Vivo, ...
We investigated the dynamics of nuclear transcription factor κB (NF-κB) by using fusion proteins of the p65 subunit with mutants of green fluorescent protein (GFP). GFP-NF-κB chimeras were functional both in vitro and in vivo, as demonstrated by electrophoretic mobility shift assays and report...
As GFP and sGFP have identical amino-acid sequences, the brighter signal from sGFP is likely to be due to a higher level of protein synthesis caused by optimal codon usage, as demonstrated by Haas et al. in the preceding paper [17]. To confirm that the selected codon usage gave higher...