GFP-Trap Magnetic Agarose: anti-GFP Nanobody conjugated to magnetic agarose beadsGFP-Trap Magnetic Particles M-270: anti-GFP Nanobody conjugated to magnetic particles M-270 for analysis of very large proteins/complexes. GFP VHH: anti-GFP Nanobody Free sample You can test the GFP-Trap yourself...
2、TurboGFP-Trap Description ChromoTek TurboGFP-Trap for Immunoprecipitation: TurboGFP-Trap is an anti-TurboGFP single domain antibody fragment, also called VHH or Nanobody, coupled to a matrix for pull down of TurboGFP-fusion proteins. TurboGFP-Trap is available as agarose (product code tbta-...
we attempted to produce a fusion nanobody with heightened affinity to GFPuv for use in purifying protein with improved yield. Repeated (GGGGS) amino sequences can form a flexible linker between two proteins, and one turn of
Here, we only showed the effect of one specific anti-GFP nanobody (GFP-booster from Chromotek) and we also acknowledge that we only tested one GFP and one EGFP variant expressed on the surface of the living cells (see Transparent methods for protein sequences). Many derivatives of GFP exis...
Plasmid Name质粒名称: POLArIS_NbVim-cp10GFP Gene/Insert插入基因名称: anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP) (Synthetic) Cat#货号: BioVector-0171020 Map图谱: Sequence序列:Contact BioVector NTCC Inc. ...
DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2. Description The Vimentin-Chromobody visualizes endogenous Vimentin in real time. • Easily transfect your cells with the Vimentin-Chromobody plasmid • Use Vimentin-Chromobody as a marker of vimentin cytosk...
Hamann M, Beschorner N, Vu X, Hauber I, Lange U, Traenkle B,et al. Improved targeting of human CD4+ T cells by nanobody-modified AAV2 gene therapy vectors. PLoS ONE. 2021;16:e0261269pubmedpublisher Luna C, Parker M, Challa P, Gonzalez P. Long-Term Decrease of Intraocular Pressure...
Sequence With the Lamin-Chromobody plasmid you receive the sequence information of the Alpaca antibody to Lamin fused to TagGFP2, as well as the full vector sequence. Transfection Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lip...
vGFP comprises GFP fused to a bound high affinity Enhancer nanobody that potentiates its fluorescence. We show that peptides inserted into the linker region between GFP and the Enhancer are correctly displayed for on-target interaction, both in vitro and in live cells by pull-down, measurement ...
After protein expression, cells are fixed/permeabilized and a solution of recombinant GFP 1–9 (GFP1–9r) with a anti-GFP nanobody (VHHr) is added and incubated for 4 h at room temperature. Following in vitro split-GFP complementation, only A/B protein complexes are visualized. (B) ...