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CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the double strand break can result in insertion or deletion mutations, while repair pathways can be engine...
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genome editingCRISPR/Cas9-based genome editing enables the rapid genetic manipulation of any genomic locus without the need for gene targeting by homologous recombination. Here we describe a conditional transgenic approach that allows temporal control of CRISPR/Cas9 activity for inducible genome editing ...
Editing endogenous genomic DNA in eukaryotic cells is now possible with CRISPR-Cas9 technology. By taking advantage of a cell’s own DNA repair mechanisms, we are able to add, change, or delete almos...
Professor Jennifer Doudna - CRISPR-Cas9 Genome Editing and the Future of Medicin 55 0 2022-06-06 21:10:174投币81https://www.youtube.com/watch?v=avz4Ca-nnLo 知识 科学科普 CRISPR/Cas Jennifer Doudna 基因编辑 昆仑断刀客 发消息 博士在读,关注干细胞及CRISPR/Cas技术,欢迎交流 Into...
More recently, the development of the molecular biological CRISPR/Cas9 system opened new possibilities for efficient and highly targeted genome editing. This chapter aims to define and consequently demystify the term "gene doping" and ... N Ewi,P Simon - 《Medicine & Sport Science》 被引量: 1...
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To accelerate the application of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/ CRISPR-associated protein 9) system to a variety of plant species, a toolkit with additional plant selectable markers, more gRNA modules, and easier methods for the assembly of one or more ...
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is a versatile and convenient genome-editing tool with prospects in gene therapy. This technique is based on customized site-specific nucleases with programmable guiding RNAs that cleave and intr...