Herein, we analyzed CRISPR knockout/RNAi knockdown screen data for over 700 cell lines and evaluated cellular dependencies of 104 AD-associated genes previously identified by genome-wide association studies (GWAS) and gene expression network studies. Multiple genes showed widespread cell dependencies ...
Lefty1andLefty2, were differentially expressed between 2i and 2iSB mESCs. However, to further understand the functional role of these two genes in 2iSB mESCs, it will be interesting to knockdown or knockout these genes and
and protection against oxidative stress [122]. Total knockout or knockdown of the Sod2 gene causes ROS-derived disorders and lipid deposition. The expression of SOD2 is decreased in the liver of NASH patients [123]. CAT and GPX preserve the liver from lipid accumulation and inflammation by re...
PCDHA protein expression in Pcdha9 mutant mice and knockdown of Pcdha9 in mice Adopted with permission from Teekakirikul et al. (2021). A–C: Immunostaining and confocal imaging show PCDHA protein expression in WT E14.5 mouse embryo aorta (A), with magnified views showing expression in smoot...
However, RNA interference knockdown ofLC3orAtg7, which are involved in autophagy, reversed the effects of LRRK2 mutation reducing autophagy in SH-SY5Y cells expressing mutant LRRK2 [83]. Additionally, 4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126), which is MAPK/ERK kin...
However, knockdown of these components promotes resistance to dasatinib treatment. The uniform abundance of individual guides in the LGR screen results in less variability after the treatment bottleneck. The screen sensitivity is improved because the read count differences between guides for a given gene...
spermatids, spermatozoa, cells of the ovary and accessory reproductive organs) can be identified by comparing transcriptomes of control and CPEB knockdown planarians. Thus, we extracted total RNA from fertile control planarians and compared it with that of CPEB1(RNAi) and CPEB2(RNAi) animals of ...
Morpholino knockdown of prdm1 did not rescue the expression of fgf8 or uncx4.1 (data not shown), indicating that additional negative regulators are involved in the loss of polarity markers in the ptc1;ptc2 mutant somites. All these data suggest that the somites of ptc1;ptc2 mutants become ...
In contrast toGPR151, knockdown ofPde3bin 3T3-L1 preadipocytes (Supplementary Fig.28a) showed no significant influence on adipogenesis and lipolysis (under either basal or β-adrenergic stimulated conditions), as compared to scRNA-transfected controls (Supplementary Fig.28b–e). SincePDE3Bis expresse...
Sterilization of the recipients generated an all-male population. Similar was reported by Slanchevet al.15, Liet al.30, Tzunget al.16, Gross-Thebinget al.17where the knockdown or knockout of thedead endgene (that is involved in maintaining the germ-line fate of PGCs17) also produced all...