A pair of previously reported primers (PPLO-F: 5′-TTACGCAAGAGAATGCTTCA-3′, PPLO-R: 5′-TAGGAAA GCACCCTATTGAT-3′) was used to detect mycoplasma (1626 bp) in samples [11]. The PCR reaction contents and conditions were the same as above. The annealing temperature was 58 °C ...
C. lanceolata PEMs consist of a clump of rounded densely cytoplasmic cells with several highly vacuolated cells [31]. In general, PEMs are transferred to the solid SE induction medium and induced to form somatic embryos [14]. However, this solid induction method is not suitable for large-sca...