The development of the cortex is a delicate balance between proliferation, differentiation, and migration of neural progenitors (NPs). Throughout developmental process, various cellular mechanisms ensure that NPs differentiate into the correct cell subtypes, migrate to their correct regions, and form the...
The HT-29 and HCT116 cells were seeded in 6 cm cell culture dishes at a density of 6 × 105 cells/dish. The following day, the cells were treated with tested fractions and incubated for 24 h. Then, the supernatant was removed, cells were washed twice with ice-cold PBS and harves...
b In the hybridization stage, HCR hybridization probes anneal to the target RNA, and adjacent probes form an initiator which allows the initiation of the HCR amplification. c In the amplification stage, HCR hairpins (h1 and h2) stay self-annealing in the absence of an initiator. When an ...
Detection of stable Cas9 activity in cancer cell lines Western blotting was performed to detect SpCas9 protein in primary liver tumor cell lines derived from CRISPR/Cas9-induced mouse liver tumors. Cells were washed with cold PBS for two times, and then lysed in Radio-Immunoprecipitation Assay buf...
For this, cells were placed in a small pre-marker area and after air drying, were fixed in 4% PFA (Sigma) for 15 min, washed three times with PBS, air dried and stored at 4 °C for H&E staining and immunofluorescence studies. Reason for collecting cells at two different speeds of ...
Microbiome alterations have been associated with depression, and fecal transfer of depressed patients’ microbiomes is sufficient to enhance despair behaviors in rodents. Yet little is known about the potential mechanisms, whereby microbes modulate depre
[68Ga]Ga-PSMA-Tz demonstrated excellent in vitro stability within 2 h with no demetallation, in PBS and serum (Additional file 1: Figure S12). In blood and urine, [68Ga]Ga-PSMA-Tz existed mainly in the form of the parent compound at 60 min p.i. (Additional file 1: Figure S13)...
To assess the effects of TPX2 and TTK depletion on the ability of CRC cells to form colonies under conditions that recapitulate the 3D tumor environment, HCT116 and HT-29 were treated with siRNAs targeting TPX2, TTK, or control siRNAs and were subsequently grown in matrigel under 3D ...
The inner layer of the retina was separated and soaked in 0.15 mol/L pH 7.4 glycine buffer overnight after being rinsed with PBS. Then, it was digested with 3% trypsin at 37 °C for 2 h. After the retina was dissolved, it was gently shaken in distilled water to wash away the inner...
For the Western blotting assay, frozen tissues were washed with ice-cold PBS, and the protein was extracted by a total extraction kit (KeyGEN, Nanjing, China). Equal amounts of proteins were separated by SDS‒PAGE and transferred to PVDF membranes (Millipore, County Cork, Ireland). Then, ...