Lu conceived the research and proposed geminal-atom catalysis. X.H. designed the experiments and synthesized the materials. Jun Li and Q.Y. conceived the Cu–Cu bonding reaction mechanism and carried out quantum-chemical calculations. N.G. and C.Z. carried out theoretical calculations of the ...
The binding reaction was carried out in 1× PBS with serial dilutions of His-BCL-2 (final concentration from 0.1 nM to 10 μM) and 10 nM FITC-labelled Bax peptide in a final volume of 150 μL. After 15 min of incubation at room temperature, the polarization in milli...
When the reaction was stopped with 2 mol/L H2SO4, the values at 450 nm were detected by an ELISA reader (Thermo Scientific, Vantaa, Finland) and endpoint titers were defined as the highest dilution of sample for which the optical density (OD) was at least twice that of the negative ...
Almeida AMR, Kiihl RAS, Almeida LA (1982) Calico mosaic of soybean: Sources of resistance and inheritance of reaction. Soybean Genet Newsl 9:18–21 Google Scholar Almeida ÁM, Amorim L, Bergamin Filho A et al (2003) Progress of soybean charcoal rot under tillage and no-tillage systems...
Samples without glucose-6-phosphate (G6P) were used to control the specificity of the reaction. Parasite lysate was prepared by sonication of Saponin (0.01%) liberated trophozoite stage P. falciparum 3D7 parasites. Briefly, P. falciparum lysate...
where the constant k is related to the adsorption capacity, and the constant 1/n represents the intensity of the reaction, with a value less than one indicating favorable adsorption. The Langmuir and Freundlich isotherms were determined for strontium concentrations of 0.1-10 mg/L and are shown ...
regulation of pH and CO2levels [191]. CAs, depending on the isoform, conditions, pH, and CO2/HCO3−ratio, may accelerate the hydration of CO2or catalyse the reaction in the opposite direction, thereby affecting the flow of CO2across cell membranes because CO2can be efficiently transported ...
Molecular and Electronic Structure of Square-Planar Gold Complexes Containing Two 1,2-Di(4-tert-butylphenyl)ethylene-1,2-dithiolato Ligands: [Au(2L)2]1+/0/1-/2-. A Combined Experimental and Computational Study From the reaction of in situ generated 1,2-di(4-tert-butylphenyl)ethylene-...
Methanomassiliicoccales are a recently identified order of methanogens that are diverse across global environments particularly the gastrointestinal tracts of animals; however, their metabolic capacities are defined via a limited number of cultured strai
Several reports demonstrated quantitative differences of SAM, which is the product of an enzymatic reaction catalyzed by MAT2A, in samples from AD patients versus controls (23, 25, 26, 27, 28). Encouraged by those findings, we next aimed to study SAM levels in our knockdown cells versus cont...