但如果将Y轴的显示切换为% MAX,那么就很容易看出绿色的峰右肩存在一个小峰的(图源FlowJo histogram overlay scaling options explained [1]): 在FlowJo较新的版本中,将直方图拖放到Layout Editor中叠加后,或者双击叠加的图,会弹出Graph Definition窗口,在该窗口中,有个Specify选项卡,其中第二个Y Axis可选择MODAL,...
Max number of gates: This limits the number of gates HyperFinder can use to identify the population, which will be important if the sorter you intend to use has a sort depth limit. F-measure beta: F-measure is the harmonic mean of precision and recall. The beta value allows the user t...
Max Sample Cache Data size (Mb) This is the maximum amount of FCS data that can be stored in memory. If exceeded, some files get booted out of memory. We suggest to set this to ~1/2 times the total RAM installed on the computer. On a PC, the default value here is set to work ...
但如果将Y轴的显示切换为% MAX,那么就很容易看出绿色的峰右肩存在一个小峰的(图源FlowJo histogram overlay scaling options explained [1]): 在FlowJo较新的版本中,将直方图拖放到Layout Editor中叠加后,或者双击叠加的图,会弹出Graph Definition窗口,在该窗口中,有个Specify选项卡,其中第二个Y Axis可选择MODAL,...
老师您好,想请教下这种图怎么做的,纵坐标设为%ofmax 这种图,在旧版的Flowjo里面,确实就是% MAX(FlowJo histogram overlay scaling options explained [1]),既能让峰图更好看,还能避免某个峰因为细胞数过少导致的细节分布看不清的弊端。不同峰的最高值都算做100%,这样的话,所有峰的细节就能都显示出来了。
Input MaxOfMeans The threshold of the average mean drift across all channels. Decreasing this value will increase the strictness of the algorithm. The Mean Drift is a measure of the quality of a given channel across all of the segments and is calculated using the mean of each of the segmen...
Before (left) and After Normalization to Max Expression of HouseKeeping Geneset Advanced Features The advanced features within this platform allow for greater control and nuance in the way parameter-sets are treated with regard to normalization. ...