但如果将Y轴的显示切换为% MAX,那么就很容易看出绿色的峰右肩存在一个小峰的(图源FlowJo histogram overlay scaling options explained [1]): 在FlowJo较新的版本中,将直方图拖放到Layout Editor中叠加后,或者双击叠加的图,会弹出Graph Definition窗口,在该窗口中,有个Specify选项卡,其中第二个Y Axis可选择MODAL,...
Set theMax Sample Cache Data size– This is the maximum amount of FCS data that can be stored in memory. If exceeded, some files get booted out of memory. We suggest to set this to ~1/2 times the total RAM installed on the computer. On a PC, the default value here is set to wo...
Advanced Scaling Recommendations Default scaling can be assigned on a per parameter basis using theadvanced parameter scalingpreferences. Each of the six image derived parameters can be assigned a unique scale, forward and side scatter can be given different ranges, and the default range for fluoresce...
For example, at the 10^0 fluorescence level, the green curve has approximately 1% of the events. %Max: This scales each curve to mode=100%. In this mode, at 10^0 bin for the green curve there are roughly half as many cells as in the mode. Manual: This will let you scale the hi...
Input MaxOfMeans The threshold of the average mean drift across all channels. Decreasing this value will increase the strictness of the algorithm. The Mean Drift is a measure of the quality of a given channel across all of the segments and is calculated using the mean of each of the segmen...
Max number of gates: This limits the number of gates HyperFinder can use to identify the population, which will be important if the sorter you intend to use has a sort depth limit. F-measure beta: F-measure is the harmonic mean of precision and recall. The beta value allows the user ...