We therefore tested whether the residual vector sequence contained in the fli1a:EGFP transgene or the integration of transgene into the genome may cause this expression 'noise' and/or deregulation of gene expression at a genome-wide level. RNA probes were synthesized using two different methods, ...
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Counterstaining the fli1a:EGFP-expressing pharyngeal arches (a1, a2) with the nucleic acid stain SYTO 59 (red) clarifies the ectomesenchymal phenotypes (double-labeled).Mark M. SasakiJames T. NicholsCharles B. Kimmel