[2].Hestand, Matthew S., et al. "Fetal fraction evaluation in non-invasive prenatal screening (NIPS)." European Journal of Human Genetics 27.2 (2019): 198-202. [3].Song, Yijun, et al. "Quantitation of fetal DNA fraction in maternal ...
胎儿DNA浓度分数(Fetal fraction,简称FF),是指胎儿胎盘来源的cfDNA占母体血浆cfDNA总量的百分比,计算公式:FF=胎儿cfDNA/(胎儿cfDNA+母体cfDNA)。在孕10-20周,FF平均值在10%-15%之间。 FF为什么对NIPT检测效能很重要? FF是影响NIPT检测性能的重要参数...
An important factor in quality control of non-invasive prenatal screening (NIPS) or testing (NIPT) is a sufficient percentage of fetal DNA to avoid false-negative results. Here we evaluate 14,379 shallow whole-genome sequenced diagnostic NIPS samples, as well as negative controls, for both ...
The fetal fraction, or percentage of total cell-free DNA (cfDNA) in a sample derived from the fetus, can impact NIPT results and profitability.
To compare available analysis methods for determining fetal fraction on single read next generation sequencing data. This is important as the performance of non-invasive prenatal testing (NIPT) procedures depends on the fraction of fetal DNA.We tested six different methods for the detection of fetal...
Yourgene Health plc, a leading international molecular diagnostics group, presented data demonstrating that prolonged storage of blood in EDTA tubes is compatible with non-invasive prenatal testing when using size selection to enrich the feta...
The purpose of this noninvasive prenatal testing (NIPT) study was to compare the fetal fraction of singleton gestations by gestational age, maternal characteristics and chromosome-specific aneuploidies as indicated by z-scores. This study was a multicenter prospective cohort study. Test data were colle...
Fig. 2: Fetal fraction amplification (FFA) increases FF for 99.8% of samples tested and most appreciably for low-FF samples. The fold-change difference in FF as a result of applying FFA is plotted for individual samples as a function of the original FF without FFA. The dashed line indicate...
Cell-free fetal DNA fraction (FF) in maternal plasma is a key parameter affecting the performance of noninvasive prenatal testing (NIPT). Accurate quantitation of FF plays a pivotal role in these tests. However, there are few methods that could determine FF with high accuracy using shallow‐dep...
Objective: It was the aim of this study to examine the possible effects of maternal and fetal characteristics on the fetal fraction in maternal plasma cell-free DNA (cfDNA) at 11–13 weeks’ gestation. Methods: In a nested case-control study, cfDNA was extracted from maternal...