A Cupheaβ-ketoacyl-ACP synthase shifts the synthesis of fatty acids towards shorter chains in Arabidopsis seeds expressing Cuphea FatB thioesterases SummaryAcyl–acyl carrier protein (ACP) thioesterases with specificities on medium chain substrates (C8–C14) are requisite enzymes in plants that ...
生物化学教学课件：lecture7 Fatty acid catabolism(脂肪酸代谢)
To achieve such a profile, Brassica napus cv. DH12075 was engineered by simultaneous seed-specific over-expression of a native fatty acyl-ACP thioesterase B [BnFatB(2)] and artificial microRNA-mediated down-regulation of eight endogenous genes encoding putative stearoyl-ACP desaturases (BnSADs)....
A specific acyl-ACP thioesterase implicated in medium-chain fatty acid production in immature cotyledons of Umbellularia californica Arch. Biochem. Biophys., 284 (1991), pp. 306-312 View PDFView articleGoogle Scholar [40] S. Mekhedov, O.M. de Ilárduya, J. Ohlrogge Toward a functional ...
Although recombinant medium-chain acyl-ACP thioesterase (TE) affects the fatty acid profile in heterologous cells, tailoring of the fatty acid composition merely by engineering a specific TE is still intractable. In this study, the activity of a C8-C10-specific thioesterase FatB2 from Cuphea ...
Simultaneous over‐expressing of an acyl‐ACP thioesterase (FatB) and silencing of acyl‐acyl carrier protein desaturase by artificial microRNAs increases ... A. Simultaneous over-expressing of an acyl-ACP thioesterase (FatB) and silencing of acyl-acyl carrier protein desaturase by artificial ...
A Cuphea beta-ketoacyl-ACP synthase shifts the synthesis of fatty acids towards shorter chains in Arabidopsis seeds expressing Cuphea FatB thioesterases. Slabaugh, A Cuphea β-Ketoacyl-ACP Synthase Shifts the Synthesis of Fatty Acids Towards Shorter Chains in Arabidopsis Seeds Expressing Cuphea FatB...
Acyl-ACP thioesterase hydrolyzes the thioester bond of acyl-ACP, releaseing free fatty acids, which are quickly transported into the cytosol via acyl-CoA synthetase in prokaryotic and eukaryotic cells [4–6]. However, such a mechanism would be detrimental to cyanobacteria, causing a loss of ...