The F-type ATPase of Escherichia coli is similar to those found in inner mitochondrial or chloroplast thylakoid membranes, and has contributed greatly to the understanding of this complicated enzyme. The catalytic site of the enzyme is in the P subunit or at the interface between the α and β...
We have shown previously that the Streptococcus mutans F-type H+-ATPase (FOF1) c subunit gene could complement Escherichia coli defective in the corresponding gene, particularly at acidic pH (Araki et al., (2013) [14]). In this study, the entire S. mutans FOF1 was functionally assembled ...
d Model of bacterial F-type-ATPase: the soluble module F1 is organized by α- (light blue), β- (dark blue), γ- (dark green), δ- (yellow) and ε-subunit (light green). Source data underlying a–c are provided as a Source Data file. Full size image The ATPase F1 part ...
The equivalent α-subunit residue, Q208, cannot function as an acid–base catalyst. The evidence for gene duplication preceding the second reversal-of-function transition (Synthase → ATPase) is even more compelling. Indeed, the atp operon of the hyperthermophilic sulfate-reducing archaeon, ...
A similar role was previously proposed for Trx in the regulation of CF1 γ-subunit of ATPase [20]. 3.5. Redox potential of AtG6PDH1 partly explains Trx specificities The redox midpoint potential (Em) of AtG6PDH1 was determined by measuring the activity of G6PDH equilibrated with different ...
[14].F-box 蛋白 TaFBA1 通过 ABA 途径提高转基因烟草的耐旱性[18].在盐胁迫 下,过表达 TaFBA1 基因烟草植株与野生型相比在 根和叶中具有更低的 Na+ 含量和更高的 K+ 含量,质膜上的 H+-ATPase 和液泡膜中 V-ATPase,PPase 的 活性也更高.由此推测 TaFBA1 的过表达提高了转 基因植株的盐胁迫...
This interaction inhibits the ATPase activity induced by Hdj-2 on HSC 70 and prolongs the interaction of CHIP with HSC70 and with the nascent CFTR peptide. Moreover, CHIP has 3 TRP domains and could bind at least 3 CFTR/HSC70/Hdj-2 complexes[64] and target all to the degradation ...
Furthermore, FAF1-mediated inhibition of TbRII expression was partially rescued by N2, N4-dibenzylquinazoline-2,4-diamine, an inhibitor of the ATPase activity of VCP (Supplementary Fig. 3b). VCP–UFD1–NPL4 as well as the E3 ligases bTRCP and CULLIN1 were found to associate with TbRII ...
β subunit of F-type ATPaseglycine-rich sequenceAffinity labeling and genetic studies on the glycine-rich sequence of the β subunit of E. coli F-type ATPase are discussed. A model of the structure of the enzyme near the γ phosphate moiety is proposed....
An activation volume of -23.7 ml 路 mol 1 was calculated for the ATPase reaction. Pressurization of the CF 0 F 1 , followed by depressurization, impairs ATPase independently of inducing a clear dissociation of catalytic subunits. This does not impair 3OMFPase activity. Addition of glycerol to ...