ELISPOTPeripheral blood mononuclear cellsPBMCInterferon-gammaIFN-γCytokinesT cellsThe quantification of single cell interferon-gamma (IFN-γ) release for assessing cellular immune responses using the Enzyme-linked immunospot (ELISPOT) assay is an invaluable technique in immunology...doi...
An Interferon-γ (IFN-γ) Enzyme-Linked ImmunoSpot (ELISpot) assay was performed to measure the magnitude of mycobacteria-specific response from historically BCG-vaccinated and BCG-naïve participants. The secretion of IFN-γ in response to purified protein derivate (PPD) was elevated in the sampl...
we developed a microfluidic device integrated with structure-switching aptamers capable of continuously tracking the concentration of the cytokine interferon gamma (IFN-γ) in cell culture medium and blood serum. First, a ferrocene (Fc)-labeled structure-switching signaling aptamer with a ...
Smaller subpools aligned in a grid were used to stimulate selected CD8+ T cells for detection of IFNγ secretion in an ELISPOT assay (R&D Systems) to identify individually recognized peptides as described.27 Reactive 15-mer sequences were compared with previously identified peptide sequences ...
To evaluate ex vivo purified protein derivative (PPD) specific Th1- and Th2-type functional responses in human tuberculosis (TB). IFN-gamma and IL-5 secreting cells were measured by a computer-assisted ELISPOT assay in the peripheral blood of patients with pulmonary TB, in patients with other...
Ex vivo IFN-γ Elispot responses after DNA immunization.Fahad, N. AlmajhdiTilo, SengerHaitham, M. AmerLutz, GissmannPeter, Öhlschläger
Ex vivo T cell IFN-γ activities by ELISpot Assay for CSP and AMA1.Ilin, ChuangMartha, SedegahSusan, CicatelliMichele, SpringMark, PolhemusCindy, TammingaNoelle, PattersonMelanie, GuerreroJason, W. BennettShannon, McGrath
Serum is not required for ex vivo IFN-gamma ELISPOT:A collaborative study of different protocols from the European CIMT immunoguiding program[J].{H}Cancer Immunology lmmunotherapy,2010,(04):619-627.Mander, A., Gouttefangeas, C., Ottensmeier, C., Welters, M. J., Low, L., van der ...
2006. HIV-Specific Cytotoxic Cell Frequencies Measured Directly Ex Vivo by the Lysispot Assay Can Be Higher or Lower Than the Frequencies of IFN-{gamma}-Secreting Cells: Anti-HIV Cytotoxicity Is Not Generally Impaired Relative to Other Chronic Virus Responses. J Immunol 176:2662-2668....
The quantification of single cell interferon-gamma (IFN-γ) release for assessing cellular immune responses using the Enzyme-linked immunospot (ELISPOT) assay is an invaluable technique in immunology. Peripheral blood mononuclear cells (PBMC) are stimula