coli derivatives were used as prey in interbacterial competition. E. coli strains DH5α and SM10 λpir were used as host strains for cloning. Bacterial strains were grown in LB broth at 37 °C with shaking and bacterial growth was monitored by optical density (OD600nm) measurement. For ...
TheE. coliDNA microarrays, containing more than 95% of the 4,290 ORFs identified in theE. coliK12 genome, were purchased at the Wisconsin Gene Expression Center, (University of Wisconsin). Prehybridization was performed at 42°C for 30–45 min in 6 × SSC, 0.5% SDS and 1% BSA, and sl...
coli K-12 chromosome at the tRNA gene leuX Upon conjugation, the transferred circularised form of the PAI II536 derivative can integrate into the recipient's chromosome. Additionally, the recipient strain SY327λpir also enables episomal replication of the transferred CI. Analysis by PCR of the...
coli BL21 (DE3) E. coli Sm10λpir Phage Sp5 Plasmids: pKD4 pKD46 pDONR201 pABB-CRS2 pDONR-ORF# Features F−, lambda−, rph-1 F−, ompT, hsdS (rB−, mB−), gal Permissive strain for replication of pABB-CSR2 Δstx2::cat-cassette ...