T cells specific for 36 WT-1 peptides were evaluable for leukemocidal activity, of which 27 (75%) lysed WT-1+ leukemic targets sharing their restricting HLA allele. Each epitope identified induced T-cell responses in most donors sharing the epitopes' presenting allele; these responses often ...
Quadriceps, liver, diaphragm, and heart tissues were harvested to quantify tissue-specific signal differences between the tested AAVs. After determining luciferase signal, tissues were processed to extract nucleic acids to determine the DNA and RNA localization in each tissue. DNA was extracted from ...
We sorted 847, 5806 and 494 antigen-specific single cells from the WA1-, Beta- and Gamma-infected groups, respectively (Supplementary Figs.4Eand Supplementary Table2). We then generated libraries using the 10x Genomics Chromium platform and recovered a total of 162, 319, and 107 paired heavy ...
Here, we isolate and purify a hemagglutinin (HA)-specific nanobody that recognizes an H7 subtype of influenza A virus. The nanobody, named E10, exhibits broad-spectrum binding, cross-group neutralization and in vivo protection across various influenza A subtypes. Through phage display and in ...
(OMVs) – rich fraction extracted from EcN-MPER culture supernatant. MPER was detected in the OMVs-rich fraction and positive control (EcN-MPER pellet) using HIV-1 gp41 (2F5) monoclonal antibody and rabbit anti-human HRP-conjugated IgG (Fc specific) secondary antibody. (C) DLS analysis ...
Any cellular immune profile can be measured in the WBA (using the 7 days cell culture supernatants) or directly in responder T cells after antigenic stimulation (in the ICS) with appropriate cytokine-specific detection systems. The choice of the cytokine test panel depends on the nature of the...
a Enhancement of antigen-specific T-cell response measured by IFN-γ production by human PBMCs stimulated with EBV peptides in the presence of indicated concentrations of APX005M for 5 days. Culture supernatants were assayed for IFN-γ in triplicate and data are shown as mean ± SEM. b...
Any cellular immune profile can be measured in the WBA (using the 7 days cell culture supernatants) or directly in responder T cells after antigenic stimulation (in the ICS) with appropriate cytokine-specific detection systems. The choice of the cytokine test panel depends on the nature of the...
4 DCs:T cells and the lowest frequency at a ratio of 1:128 DCs:T cells. While we used a similarly high ratio of DCs:T cells in our expansion protocol (1:10 DCs:T cells), we could have further increased this ratio to expand an observable antigen-specific population against ERV-K-...
The MACS-enriched fraction was submitted to limited-dilution culture, and 3 different p635-649–specific CD4+ T-cell clones and 10 lines could be established. The fraction of CD4+ T cells was less than 80% before MACS separation and ranged from 81% to 90% in the various T-cell lines ...