in a graded alcohol series, and immunostained with an antibody against proliferating cell nuclear antigen (PCNA), a marker for dividing cells, using a commercial kit from Invitrogen (Carlsbad, CA) followed by counterstaining with hematoxylin as previously described (Halevy et al., 2000, 2001). ...
Development of the posterior lateral line (PLL) system in zebrafish involves cell migration, proliferation and differentiation of mechanosensory cells. The PLL forms when cranial placodal cells delaminate and become a coherent, migratory primordium that
We validated the specific expression in the primordium of a subset of the identified sequences by quantitative RT-PCR, and by in situ hybridization. We also show that interfering with the function of two genes, f11r and cd9b, defects in primordium migration are induced. Finally, pathway ...