ProtocolsforNeuralCellCulture,3rdEd.•Ed.:S.FedoroffandA.Richardson•HumanaPress,Inc.,Totowa,NJ 95 ChapterEight PrimaryCellCulturesfortheStudy ofMyelination MaryI.Johnson,RichardP.Bunge,andPatrickM.Wood DEDICATION ThischapterisdedicatedtoRichardP.Bunge(1932–1997),whosecontributionswereinnu- merableto...
University of Arizona Cell Culture-893细胞;细胞背景资料:详见相关文献介绍;细胞传代方法:1:2-1:3传代;每周换液2-3次。;细胞生长特性:贴壁生长;细胞形态特性:上皮样;相关细胞有:GM2132细胞、SKBr3细胞、Sp2/O细胞 JHH2细胞;细胞背景资料:肝癌;腹水转移;男性;细胞传代方法:1:2-1:3传代;每周换液2-3次。;...
Discussion: Primary DRG cell cultures represent an efficient way to examine pain pathways. Using target specific inflammatory stimuli, we were able to demonstrate that a TRPV1 agonist (e.g. capsaicin) stimulates DRG expression of peptide neurotransmitters (e.g. Substance P), and that there are ...
A cell culture model could be used to study human tau pathology and screen for drugs to treat tauopathies. Further details on the research, next steps and licensing status are discussed in the article.SciBX: Science-Business eXchangedoi:10.1038/scibx.2014.34None...
DRGcellcultureswere exposed toCAPorRTXfor 10minutesat3 daysof culture age thenculturedinfreshmediaforanother6 days.SP andTRPVl protein 5 ,、:,口《{l《roj,‘§jl■ql 山东大学硕士学何论文 expression were investigatedby Westernblot.SP expression ...
Our understanding of the processes controlling peripheral nervous system myelination have been significantly benefited by the development of an in vitro myelinating culture system in which primary Schwann cells are cocultured together with primary sensor
网络背根神经节(dorsal root ganglion);数字栅格地图(Digital Raster Graphic);背根节 英汉 网络释义 n. 1. 诊断相关级 例句 释义: 全部,诊断相关级,背根节 更多例句筛选 1. meanwhile, Schwann' s cell and neuron migrated along these neurons. The neurons, dissociated from DRG, grew well on the surfa...
For monitoring cell migration MDA-MB-231 cells were co-transfected with EsuDRG1-FLAG or HsaDRG1-FLAG and EsuDFRP1-MYC or HsaDFRP1-MYC. The cells were seeded in a migration Transwell cell culture inserts (pore size 8 mm; Corning) at a density of 2.5 × 104 cells/well, and left...
Cell culture DRG cultures were prepared as described previously [25]. Briefly, P2 rat pups were put on ice and then decapitated to harvest DRG. DRG were then dissected out under microscope and dissociated with 0.25% trypsin and 0.05% collagenase (Sigma) in HBSS solution, for 30 min at 37...
Experiments with the tissue chips have produced more detailed information than tests using animal or cell models. NCATS just announced＄17 million in grants(拨款)to develop an entire human organ system over the next three years. 13.Why are scientists designing silicon computer chips acting like ...