Comparative analysis of genomes is valuable to explore evolution of genomes, deduce gene functions, or predict functional linking between proteins. Here, we have systematically analyzed the genomic environment of all known DNA replication genes in 27 arc
SENAX can assemble up to 6 DNA fragments To evaluate the performance of SENAX in assembling multiple fragments, we performed assembly using constructs of varying sizes (2.8 kb and 10 kb) (Figs. 1b, 2) and with a varying number of joining fragments (3, 4, 5, 6 and 7) (Fig. 2)....
A nucleosome is the fundamental repeating unit of the eukaryotic chromosome. It has been shown that the positioning of a majority of nucleosomes is primarily controlled by factors other than the intrinsic preference of the DNA sequence. One of the key qu
ESCs),是一种在胚胎阶段从囊胚内细胞团中分离出来的,具有分化为除胎盘和脐带以外的任何身体细胞类型的多能性干细胞[2];②成体干细胞 (adult stem cells,ADCs),是一种可以分化成其所在细胞谱系中的特定细胞类型的多能干细胞[3];③诱导多能干细胞 (...
DNA methylation has been documented to play vital roles in diseases and biological processes. In bovine, little is known about the regulatory roles of DNA methylation alterations on production and health traits, including mastitis. Here, we employed whol
Because Mecp2 is known to specifically bind methylated cytosines, we analyzed the direction of the methylation change of Mecp2 target dmCpGs with aging and ENR. The vast majority of Mecp2 targets (87.31%) were hypomethylated with aging and hypermethylated by ENR (Fig. 3d). Aging-induced hy...
Wedderburn2,3,9, Kirsten A. Donald2,3, Michael S. Kobor10,11,12, Heather J. Zar2, Dan J. Stein3,4,5 and Anke Hüls1,13* Abstract Background Exposure to indoor air pollution during pregnancy has been linked to neurodevelopmental delay in toddlers....
In the study of transposable elements (TEs), the generation of a high confidence set of consensus sequences that represent the diversity of TEs found in a given genome is a key step in the path to investigate these fascinating genomic elements. Many algo
DNA replication and cell cycle regulation are frequently disrupted as part of a cancer’s progression toward uncontrolled proliferation1,2,3. The resulting dysregulation creates a positive feedback loop of increasing replication stress and genomic instability4,5,6, generating new somatic copy number alt...
We further validated our meQTLs by performing independent external replication analysis based on 963 EA samples from the ARIC study and 384 AA samples from GTP. We found that more than 99% ofcis-andtrans-meQTL SNP-CpG pairs showed consistent allelic direction of effect in relation to methylatio...