目的观察病毒基因组5′端非编码区内与翻译起始有关的位点和结构 蛋白编码区的特异性反义核酸对病毒蛋白表达的影响及其量效关系.方法应用病毒致细胞病变作用保护实验、空斑形成实验和空斑形成减少实验、Western blot实验等方法,观察特定的反义核酸抑制病毒感染的效果.结果针对IRES位点、AUG区域和VP1区的3条反义核酸Scb...
摘要 目的观察病毒基因组5′端非编码区内与翻译起始有关的位点和结构蛋白编码区的特异性反义核酸对病毒蛋白表达的影响及其量效关系.方法应用病毒致细胞病变作用保护实验、空斑形成实验和空斑形成减少实验、Western blot实验等方法,观察特定的反义核酸抑制病毒感染的效果.结果针对IRES位点、AUG区域和VP1...
we had previously reported that the pBRCVB3 virus induces mainly pancreatitis, but its myocarditis-inducing ability was severely impaired11. Using this virus as the backbone, we sought to create attenuated mutant viruses that would lose their ability to induce both myocarditis and pancreatitis, thus ...
we had previously reported that the pBRCVB3 virus induces mainly pancreatitis, but its myocarditis-inducing ability was severely impaired11. Using this virus as the backbone, we sought to create attenuated mutant viruses that would lose their ability to induce both myocarditis and pancreatitis, thus ...