CRYOELECTROCOAGULATORMKHITARYANTS ARAKEL M,SUSANDOMIRSKIJ BORIS P,SUPISANNYJ OLEG E,SU
The fluorescent markings served for easy navigation for the following cryo electrotomography (cryo ET). Klein et al., Commun Biol. 2021 Jan 29;4(1):137 [11] investigated lamellar bodies (LBs) and found an undescribed outer membrane dome protein complex (OMDP) to be limiting membrane of LBs...
While vibration, noise and electro-magnetic interference (EMI), from both alternating (AC) and direct (DC) current sources are enemies of microscope performance, contamination and excess humidity also diminish performance. Assuming that a careful site selection has eliminated or mitigated some of these...
Each purified sucrose gradient fraction (40%, 50% and 70%) was centrifuged (15.000 g, 4 °C, 15 min), the pellet was resuspended and boiled in Laemmli sample buffer, and then resolved by gel electropho- resis. Entire gel lanes were excised manually from Coomassie blue-stained ...
To generate cell lines for nanobody-free GEM2 labeling, HeLa cells were electroporated with four plasmids, 5 µg each: the same two Cas9–sgRNA-expressing plasmids as above, GEM-Halo-FKBP AAVS1 donor plasmid with a puromycin resistance gene and Mito-mCherry-FRB AAVS1 donor plasmid ...
Electrohydrody... MK Kim,KH Lee - 《Polymer Korea》 被引量: 0发表: 2014年 Improving hemocompatibility in tissue-engineered products employing heparin-loaded nanoplatforms Silk FibroinCTComputed TomographyDVTDeep Vein ThrombosisDOCADeoxycholic AcidDSDermatan SulfateDMAcDimethylacetamideDOXDoxorubicinEHD...
Electro Lymphatic Circulation is designed to improve sluggish lymphatic systems, swelling in limbs, or those not seeing results quickly. Lymphatic circulation is a great way to release dead fat cells out of the body faster. Get More Information Testimonials My initial reason for doing Cryo was to...
5e6 T-cells were suspended in 100 μl nucleofection buffer (Lonza VPA-1002) containing 30 μg Cas9 complexed with 150 pmol of each sgRNA, and electroporated with the T-020 program on the Lonza Nucleofector IIb. Cells were transferred immediately into media containing adeno-associated virus (...
The synthetic DNA fragment was used as the donor DNA and was electroporated into C. jejuni 11168 by using a Bio-Rad GenePulser Xcell Electroporation System (Hercules, CA). Transformants were selected on Mueller-Hinton (MH) plates containing 15 μg/mL of chloramphenicol and were confirmed ...
Cryo - The Portal (Electro Spectre Remix)