The top-producing clones were counted and passaged at least two times into TPP tubes containing 15 mL of GS selection medium before starting the tertiary screen to ensure that the doubling times have stabilized. Tertiary Screening. To identify the best producing clones from each group for the ...
Results from DEG testing on screen data and on swap rate simulations of data Capture rate / MOI parameter sweeps with log likelihoods Intermediate data files for our tests for enrichment of genotypes in TSNE clustersThis is a much smaller download at 2.8GB and is probably of greater utility to...
The genotype of all 52 mutant larvae were further analysed using methods commonly reported in the literature to screen CRISPR/Cas9 efficacy. Regression analysis between mutation rates determined by NGS and the arbitrary gene modification rates assessed by T7E1, RGEN and fragment analysis, revealed a ...
For knock out of CIITA, IDAA was used as a screen tool. Knockout was confirmed by sequencing (Supplementary Fig. 1C). No off-targets were detected for any of the three edits from sequencing the top 5 off-target sites (Supplementary Fig. 2). Pluripotency of the edited cell line ...
Nevertheless, these GEMMs provide a solid platform to screen and test for new therapies, including immunotherapy, that have the potential to improve current standard treatments. The improvements in genome editing via the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated ...