Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essent
were used for immunoblot with anti-DDX1 (NovusBiologicals or ProteinTech), anti-AID (Cell Signaling L7E7), anti-Tubulin (Sigma-Aldrich), anti-Actin (Sigma-Aldrich), anti-CTCF (Millipore) or anti-Histone H3 (Abcam) and HRP-conjugated secondary antibodies in TBST, 5% milk (Sigma-Aldrich). ...