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It has recently been published that netrin 1 and netrin 4 inhibit the activity of the endothelial cells by means of the receptor UNC5H2 (Lu et al., 2004) and inhibit the vascularization of the retina. The purpose of the present invention is also to provide novel anti-angiogenic agents. Th...
Female athymic nude mice (6-8 wk/old) were xenografted subcutaneously with 1×106 cancer cells. Mice were euthanized with CO2 three weeks post-graft, the tumors weight being within the 300-650 mg. Peripheral blood was drawn into EDTA tubes and was immediately (within one hour) used for pl...
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The amino acid sequence of human netrin-1 or human netrin receptor such as UNC5H1, UNC5H2 and UNC5H3 (Unc-5 homolog 1, 2 and 3 equivalent to Unc-5 homolog A, B and C) are well known by the skilled man. Example of these amino acid sequences with the localization of their particul...
Obtained as a white solid (412 mg, 45%) following Method A: 8 (968 mg, 2.00 mmol), Pd/C 10% (96.8 mg), in distilled CH2Cl2 (30 mL). The mixture was worked up, aqueous layer was extracted with CH2Cl2 and the crude product was purified on silica gel (0 to 5% MeOH in CH2Cl2)...
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