Furthermore, they have an enviable record of performance and safety with the salient advantage that they can be easily reversed using hyaluronidase should unwanted placement or an adverse event occur. This chapter will review the FDA pivotal trial data on safety and efficacy for each approved ...
15 HA's enzymatic degradation occurs through the involvement of certain enzymes including hyaluronidase (hyase), β-N-acetyl-hexosaminidase, and b-d-glucuronidase. Hyaluronidase acts by cleaving HA that has a high molecular weight (MW) into smaller oligosaccharides, while the oligosaccharide can be ...
The skin epidermis is continually influenced by a myriad of internal and external elements. At its basal layer reside epidermal stem cells, which fuels epidermal renovation and hair regeneration with powerful self-renewal ability, as well as keeping diverse signals that direct their activity under sur...
Adenoviruses (AdVs) used for clinical applications are mainly represented by serotypes 5 and 2 (AdV5 and AdV2) [87,88,89]. Structurally, AdVs are nonenveloped viruses with linear double-stranded DNA genomes ca. 40 kb in size [90]. Targeted genetic modifications of viral genomic regions in...
Clinical applications of epidermal stem cells Since the initial cultivation of human skin stem cells in vitro and their successful engraftment in murine models [150,151,152], EpSCs have become a focal point in research and clinical applications for wound management and other skin disorders, thanks...
thereby expanding the applications of electrospinning [21]. To facilitate the delivery of BAPPs, core solutions can be introduced during electrospinning to mitigate initial burst release. For example, Evrova et al. [208] incorporated PDGF-BB into the core phase of coaxial electrospun fibers aimed ...
14.Hyaluronidase treatment reveals a different gene expression profile of circulating tumor cells enriched with antibody based methods in prostate cancer patients 机译:透明质酸酶治疗揭示了富含抗体基于前列腺癌患者的抗体方法的循环肿瘤细胞的不同基因表达谱 作者:Josefsson A.;Larsson K.;Damber J. E.;Kubist...
The tissues were washed with sterile 1×PBS, minced with surgical scissors, and digested in a solution containing collagenase I, III, and IV (0.5-1 mg/mL), hyaluronidase (0.05–0.1 mg/mL), DMEM/F12, 10 mM HEPES, 2% BSA, 0.48 µg/mL hydrocortisone, 5 µM Y-27,632, and 50 ...
hyaluronidase, an enzyme that transiently breaks down hyaluronic acid, decreasing the viscosity of the extracellular matrix and facilitating DNA diffusion [135]. For antibody gene transfer, this led to an increase in mAb expression by approximately 3.5-fold, achieving plasma peak titers of 3.5 µg...
Extensive preclinical experiments and clinical trials in the realm of pancreatic cancer have provided valuable insights into the complexities of immunotherapy. Chief among the hurdles are the immunosuppressive tumor microenvironment, limited immunogenicity, and the inherent heterogeneity of pancreatic cancer. ...